TABLE 1

Primers and probes targeting the investigated viruses in sewage and patient samples

VirusSequence of:Reference or source
Forward primerReverse primerProbe
Norovirus GIYTGGATGCGNTTYCATGACTTAGACGCCATCATCATTAGATYGCGATCYCCTGTCCAAGATCGCGGTCTCCTGTCCAModified from reference 73
Norovirus GIITGGAYTTTTAYGTGCCCAGCGACGCCATCTTCATTCACAGCCAGATTGCGATCGCCC74
AstrovirusTCTTCTCAACGTGTCCGTATCTAATCAACGTGTCCGTATTTTGGTCCTGTGACACCATTGTCAATAARCAACTCAGGAACAThis study
AdenovirusCTCGACATGACTTTTGAGGTGTAGACGGCCTCGATGACAGCCCACACTTCTModified from reference 75
Aichi virusTGGGCTGCCATGTACAAGAGCCGTTGACGGTCACCACCCACTCCATGTGGAATTGCThis study
HAV (qPCR)AAAGTGGAGTTTASTCAGTGTAAAGTGGAATTYACTCAGTGTAAAGTGGAATTTACTCAATGTACWGCAGGAAAATTAATCATGTGAATGTGGTCTCCAAAACGCTGAATGTAGTCTCCAAAACGCThis study
ParechovirusATGGCGTGCCATAACTCTAATGGCGTGCCATAATTCTAAGAGGCACCTGTTACCAGAGGGGCACCTGTTACCACAGAGGCATCTGTTACCAGCTTATGCTCACACAGCCATCCTCTThis study
RotavirusACCATCTACACATGACCCTCACCATCTTCACGTAACCCTCGGTCACATAACGCCCATGAGCACAATAGTTAAAAGCTAACACTGTCModified from reference 76
HEVGGTGGTTTCTGGGGTGACACGCGGCACACGATCAGAGGGGTTGGTTGGATGAAGGGGTTGGTTGGGTGAATGATTCTCAGCCCTTTGATTCCCAGCCCTTThis study
HAV (PCR for sequencing first PCR)AAYGTTGCTTCYCATGTYAGRGTACCADGCCATDCCATCHACATC77
HAV (nested PCR)ATGYTTTGCWCCHCTHTATCATACCADGCCATDCCATCHACATC77