TABLE 3

MICs and MBCs of the four EOCs against L. monocytogenes Scott A in different concentrations of ethanol at 32°C and 21°C determined using the broth dilution method (n = 4)

CompoundEthanol concn (%)MIC (g/liter)bMBC (g/liter)b
32°C21°C32°C21°C
Thymol<1.3a0.20.20.30.3
2.50.20.20.30.3
50.2<0.05c0.30.3
Carvacrol<1.3a0.20.20.30.3
2.50.20.20.30.3
50.2<0.05c0.30.3
Eugenol<1.3a0.80.51.31.3
2.50.70.41.11.1
50.5<0.2c1.01.0
trans-Cinnamaldehyde<1.3a0.20.21.41.4
2.50.20.11.21.2
50.2<0.05c0.90.9
Ethanol10%5%20%20%
  • a The concentration derived from the stock solution with 95% aqueous ethanol to prepare an overall EOC concentration of 1.4 g/liter.

  • b MIC is in g/liter for all compounds except ethanol.

  • c Below the lowest concentration used in the assay.