TABLE 4

Relative protein abundances of the enzymes metabolizing pyruvate during sulfate respiration and pyruvate fermentation by D. alaskensis G20 and CycA mutant I2a

ReactionLocusProtein nameAnnotationcLactate-sulfatedPyruvatee
I2 abundanceG20 abundanceLog-2Rf (I2/G20)I2 abundanceG20 abundanceLog2R (I2/G20)
AbDde_0182GlcD(S)-2-Hydroxy acid oxidase7.306.520.160.42NRgIh
Dde_0312COG-GlcDFAD/FMN-containing dehydrogenase11.139.230.272.466.74−1.45
Dde_0750LdlDLactate dehydrogenase14.5811.820.3020.0911.740.77
Dde_1087COG-GlcDFAD/FMN-containing dehydrogenaseNRNRNDiNRNDND
Dde_3238LldPl-Lactate permease4.234.120.041.041.32−0.32
Dde_3239GlcDGlycolate oxidase445.77441.430.01407.13508.48−0.32
Dde_3240LdhBLactate dehydrogenase34.2231.510.1219.3411.130.79
BDde_2081PycPyruvate carboxylase21.6621.050.046.706.320.08
CDde_1253COG-SfcAMalate:NADPH oxidoreductase, decarboxylating0.4040.38−6.672.4147.73−4.31
Dde_3637TmeMalic enzyme8.9311.63−0.38ND53.20Gj
DDde_0240FumCFumarate hydrataseNDNDNDNDNDND
Dde_1254COG-FumAFumarate hydratase, β subunitND30.30GND28.20G
Dde_3638COG-FumAFumarate hydratase, β subunitNDNRNDNRNDND
Dde_3639COG-TtdAFumarate hydratase, α regionND0.26GNRNDND
EDde_1256FrdBFumarate reductase, FeS proteinND26.64GNR22.28G
Dde_1257FrdAFumarate reductase, flavoprotein subunit6.3827.15−2.09NR27.28G
Dde_1258FrdCFumarate reductase, cytochrome b subunitND12.55GND34.69G
FDde_0473FhcAPutative cytoplasmic formate:hydrogen lyase, formate dehydrogenase subunitNDNDNDNDNDND
Dde_0474FhcBPutative cytoplasmic formate:hydrogen lyase, FeS subunit 1NDNDNDNDNDND
Dde_0475FhcCPutative cytoplasmic formate:hydrogen lyase, hydrogenase, small and large subunitsNDNDNDNDNDND
Dde_0476FhcDPutative cytoplasmic formate:hydrogen lyase, FeS subunit 2NDNDNDNDNDND
Dde_0679FdoGFormate dehydrogenase, sulfur transferase0.680.74−0.1111.374.341.39
Dde_0680FdoIFormate dehydrogenase, cytochrome B556NDNDNDNDNDND
Dde_0681FdoHFormate dehydrogenase, FeS cluster2.852.570.153.07NDI
Dde_0716Formate dehydrogenase, TAT pathway signal35.290.486.21114.55NDI
Dde_0717FdhA-1Formate dehydrogenase, α subunit46.972.044.53119.830.338.57
Dde_0718FdhB-1Formate dehydrogenase, β subunit41.101.804.51143.51NRI
Dde_0812FdhB-2Formate dehydrogenase, β subunitNDNDNDNDNDND
Dde_0813FdhA-2Formate dehydrogenase, α subunit22.38NRI128.51NRI
Dde_3513FdhA-3Formate dehydrogenase, α subunitNR0.36G9.62NDI
Dde_3514FdhB-3Formate dehydrogenase, β subunitNDNDNDNDNDND
GDde_3028CooSCarbon monoxide dehydrogenase4.881.212.0297.485.384.18
Dde_3029CooCCarbon monoxide dehydrogenase4.660.942.3226.272.123.63
HDde_1792PorAPyruvate-ferredoxin- oxidoreductaseNR1.44G11.716.470.86
Dde_1793PorBPyruvate-ferredoxin- oxidoreductase3.083.040.028.177.740.08
Dde_3237PorPyruvate-ferredoxin- oxidoreductase232.69224.030.05118.14116.820.02
IDde_3241PtaPhosphotransacetylase69.0667.970.0221.8826.32−0.34
JDde_3242AckAAcetate kinase70.6688.75−0.3327.0227.070.00
  • a The data presented here are supporting data for Fig. 3. The protein abundance values given in Fig. 3 were chosen for the most abundant isozyme for that reaction, and the locus, protein name, annotation, and data are highlighted in bold.

  • b A through J designate the reactions illustrated in Fig. 3.

  • c Protein annotation was obtained from http://www.microbesonline.org. FAD, flavin adenine dinucleotide; FMN, flavin mononucleotide; TAT pathway, twin-arginine translocation pathway.

  • d Lactate-sulfate was MO basal salts with 60 mM sodium lactate and 30 mM sodium sulfate for sulfate respiration.

  • e Pyruvate was MO basal salts with 60 mM sodium pyruvate for growth by fermentation.

  • f R, I2/G20 relative protein abundance values.

  • g NR, data did not meet the criteria for reproducibility.

  • h I, log2 R was not meaningful since protein was detected only in the I2 culture.

  • i ND, no protein was detected in either G20 or I2 or the data did not meet the confidence criteria.

  • j G, log2 R was not meaningful since protein was detected only in the G20 culture.