TABLE 3

NEM modification of suicin and its mutants

Suicin designationCalculated molecular mass (Da)Molecular mass by MSPTMaNEM addition (Da)Molecular mass Δ (Da)bNo. of NEM
WT3,379.633,341.282H2O + S-S3,593.55252.272
T8A/T10A3,319.613,319.44c3,819.87500.434
C13A3,347.663,309.472H2O3,561.55250.082
C21A3,347.663,311.522H2O3,436.58125.061
C30A3,347.663,311.532H2O3,436.60125.071
C33A3,347.663,309.522H2O3,561.64250.122
T10A/C33A3,317.653,299.641H2O3,549.69250.052
  • a PTM, posttranslational modification.

  • b Data represent the mass difference after NEM addition.

  • c –, no modification.