Steady-state kinetic parameters of HMFOa

  • a The kinetic parameters of HMFO are determined in a 50 mM potassium phosphate buffer (pH 8.0) at 25°C with REDFLASH sensor spots and a Firesting O2 detector and light source on all substrates unless stated otherwise.

  • b Kinetic parameters were determined by H2O2 formation in a coupled assay.

  • c Kinetic parameters were calculated from the increase in the product's absorbance at 340 nm.

  • d ND, not determined.