TABLE 2

Comparison of the properties of type III pullulan hydrolases

PropertyResult for pullulan hydrolase type III from:
T. kodakarensis KOD1T. aggregans
Molecular mass (kDa)84.480
Catalytic subunitsMonomerND
Optimum temp (°C)95–10095
Activity at 120°C (%)>60>35
pH range3.0–8.53.5–8.5
Activity at pH 3.5 (%)10050
Optimum pH for activity3.56.5
Ca2+ requirementNoNo
Half-life at 100°C (min)4590
Km (mg/ml) for pullulan2.02.38
Vmax (U/mg) for pullulan109.1716.6
Complete hydrolysis of pullulan10 min16 h
End products of pullulan hydrolysisMaltotriose, panose, maltose, isomaltase, and glucoseMaltotriose, panose, maltose, and glucose
Hydrolysis of glycogenYesNo
Hydrolysis of cyclodextrinsAll typesOnly β- and γ-cyclodextrins
Smallest oligosaccharide hydrolyzedMaltotrioseMaltotetraose