TABLE 2

Prokaryotic diversity indices calculated based on a cutoff of 97% similarity of 16S rRNA gene sequences and 5,652 reads per samplea

Cellar age (yr)Chao1Observed OTUShannon index
1403.2 ± 72.1ac247.2 ± 18.0ac3.26 ± 0.55a
10335.1 ± 33.1bc221.2 ± 17.3a4.70 ± 0.74b
25457.1 ± 62.0a287.5 ± 19.2b5.61 ± 0.16c
50413.3 ± 69.5ac275.0 ± 33.8bc5.50 ± 0.30c
  • a All data are presented as means ± standard deviations (n = 5). Values with different letters in a column mean significant differences at P < 0.05 as determined by ANOVA.