TABLE 1

Oligonucleotide primer and probe sequences for TaqMan real-time RT-PCR assay of AdV2 and MNV

VirusPrimer or probeName (position, 5′→3′)SequenceReference
AdV2Forward primerJHKXF (18891–19910)5′-GGA CGC CTC GGA GTA CCT GA-3′27
Reverse primerJHKXR (19025–19007)5′-CGC TGI GAC CIG TCT GTG G-3′
ProbeJHKXP (18939–18960)5′-FAM-CAC CGA TAC GTA CTT CAG CCT G-MGB-3′
MNVForward primerMNV1 F (5614–5630)5′-ACG CCA CTC CGC ACA AA-3′26
Reverse primerMNV1 R (5649–5657)5′-GCG GCC AGA GAC CAC AAA-3′
ProbeMNV1 P (5632–5646)5′-VIC-AGC CCG GGT GAT GAG-MGB-3′