TABLE 1

Primer sequences used in this study and predicted amplicon sizes

Primer nameSequence (5′–3′)Predicted amplicon size (nt)a
16S ForGGT CAA GAC TGA CGC TGA GTC A136
16S RevGGC GGC ACA CTT AAC ACG TTA G
flaB ForGGG TCT CAA GCG TCT TGG139
flaB RevGAA CCG GTG CAG CCT GAG
ospA ForATG AAA AAA TAT TTA TTG GGA ATA GG829
ospA RevATT CTC CTT ATT TTA AAG CG
ospC ForbATG AAA AAG AAT ACA TTA AGT GC638
ospC RevCTT AAT TAA GGT TTT TTT GG
p66 ForGAT TTT TCT ATA TTT GGA CAC AT755
p66 RevTGT AAA TCT TAT TAG TTT TTC AAG
16S IGS ForAGT GCG GCT GGA TCA CCT CC950
ileT IGS RevbGTC TGA TAA ACC TGA GGT CGG A
nid1 ForCCA GCC ACA GAA TAC CAT CC153
nid1 RevGGA CAT ACT CTG CTG CCA TC
recA ForGTG GAT CTA TTG TAT TAG ATG AGG CT171
recA RevGCC AAA GTT CTG CAA CAT TAA CAC CT
I. scap 16S ForCGG TCT GAA CTC AGA TCA AG300
I. scap 16S RevGGG ACA AGA AGA CCC TAT C
MLST primers for amplification and sequencingc
    clpA ForAAA GAT AGA TTT CTT CCA GAC982
    clpA RevGAA TTT CAT CTA TTA AAA GCT TTC
    clpX ForGCT GCA GAG ATG AAT GTG CC884
    clpX RevGAT TGA TTT CAT ATA ACT CTT TTG
    nifS ForATG GAT TTC AAA CAA ATA AAA AG1,049
    nifS RevGAT ATT ATT GAA TTT CTT TTA AG
    pepX ForACA GAG ACT TAA GCT TAG CAG811
    pepX RevGTT CCA ATG TCA ATA GTT TC
    pyrG ForGAT TGC AAG TTC TGA GAA TA801
    pyrG RecCAA ACA TTA CGA GCA AAT TC
    recG ForCCC TTG TTG CCT TGC TTT C805
    recG RevGAA AGT CCA AAA CGC TCA G
    rplB ForTGG GTA TTA AGA CTT ATA AGC760
    rplB RevGCT GTC CCC AAG GAG ACA
    uvrA ForGAA ATT TTA AAG GAA ATT AAA AGT AG911
    uvrA RevCAA GGA ACA AAA ACA TCT GG