qPCR results for selected genes

Locus IDaJ2315 orthologbDescriptionaFold change for mutant vs wtc
I35_6025BCAM2143Large protein BapA−1.8 ± 0.2
I35_4183BCAM0185Lectin BclC−2.7 ± 0.2
I35_7309BCAS0293Nematocidal protein AidA−3.2 ± 0.2
I35_4767BCAM0854Glycosyltransferase−5.6 ± 0.5
I35_4923BCAM1004GDP-mannose 4,6-dehydratase−4.8 ± 0.2
I35_0767BCAL3106Urease subunit−84.4 ± 30.1
I35_2935BCAL0730Ammonium transporter−1,277 ± 200
  • a Nomenclature and descriptions are given according to data in GenBank (accession no. HG938370, HG938371, and HG938372).

  • b Orthologs were identified as described in Materials and Methods.

  • c Fold change in qPCR expression for comparison of the σ54 mutant and the wild type (wt) grown in minimal medium in a shift experiment.