TABLE 1

Test sites for discriminant analysis within the 5S-23S rRNA intergenic spacer region used to determine the genospecies for B. burgdorferi sensu lato

GenospeciesSite sequence for test no.a:
123456
B. afzelliiTTAAATAAAACAAATG
TA-AAGTTTTAAAA—
B. gariniiTATG-GGTTTATGGTTTTAAAA—G
TATATTGTTCATTAAAACAAAAA-T
GTTCATGAAAA–
ATTTATG
B. burgdorferi sensu strictoAT-GTAATTGGTGGGGTTGAAAA-TC
GTGTTGAAA—T
B. valaisianaTATATAGTTCATGGTTTTAAAA—G
TATGTGTTTATGATTTAAAAAA-TT
T—-ATTCATG
–TAT
  • a Figure S5 in the supplemental material shows the positions of the test sites. Variation at test sites is from 100 randomly selected sequences of each genospecies selected from the NCBI database (more common sequence polymorphisms are in boldface).