TABLE 1

Mutant strains constructed and/or used in this study

A. vinelandii strainPlasmid utilizedGenetic featuresaParent strain
DJbNoneWild type
AZBB010pPCRNH3-15ΔnifLA::StrrDJ
AZBB020pPCRNH3-14ΔnifLA::nifAAZBB010
AZBB023pPCRSCRK31ΔscrX::lacZ-KanrDJ
AZBB030pPCRURE3ΔureABC::StrrDJ
AZBB035pPCRURE3ΔureABC::Strr and ΔnifLA::nifAAZBB020
AZBB063pPCRNH3-21ΔnifLA::Tetr and ΔscrX::lacZ-KanrAZBB023
AZBB085pEB001amtB::Kanr-transposon and ΔureABC::Strr and ΔnifLA::nifAAZBB035
AZBB086pEB001amtB::Kanr-transposon and ΔureABC::Strr and ΔnifLA::nifAAZBB035
AZBB088pEB001amtB::Kanr-transposon and ΔureABC::StrrAZBB030
AZBB091pEB001amtB::Kanr-transposon and ΔureABC::StrrAZBB030
AZBB093pEB001amtB::Kanr-transposon and ΔureABC::Strr and ΔnifLA::nifAAZBB035
AZBB094pEB001amtB::Kanr-transposon and ΔureABC::Strr and ΔnifLA::nifAAZBB035
AZBB102pPCRAMTBK3ΔamtB::KanrDJ
AZBB103pPCRAMTBK3ΔamtB::Kanr and ΔureABC::StrrAZBB030
AZBB148pPCRNH3-42nifL::Kanr similar to MV376cAZBB010
AZBB150pPCRNH3-43nifL::Kanr incorporated slightly upstream of SmaI siteAZBB010
AZBB158NoneSpontaneous mutation of AZBB148 resulting in Nif+ phenotypeAZBB148
AZBB163NoneSpontaneous mutation of AZBB150 resulting in Nif+ phenotypeAZBB150
  • a Tetr, tetracycline resistance; Strr, streptomycin resistance; Kanr, kanamycin resistance.

  • b Setubal et al. (35).

  • c Brewin et al. (8).