TABLE 3

Primers used in this study

PrimerSequence (5′–3′)aPurpose
BBP919GACTAGAA TTCGTGCAGA AACATCTCTA CCCGGAAGureABC gene and flanking region cloning
BBP920GACTAAAG CTTGGAACAG AAGACGATGA GGATGCureABC gene and flanking region cloning
BBP938GACTAGGA TCCGTGAAGA TCAGCAGCTT GTCTTTCTCureABC gene deletion
BBP939GACTAGGA TCCAAGAAGG ACCTGATCCA CAACGureABC gene deletion
BBP1147GAGCAAGC TTCATGGTCA GGTCGTGGCC TTCnifLA gene and flanking region cloning
BBP1148GACAGGAT CCGGTCAGCA CTTCGGACAC CACnifLA gene and flanking region cloning
BBP1209GACATCTA GACTCTCATA TGGTGCCTCG TCTATCCAAG AAAACCnifLA gene deletion
BBP1210GACATCTA GAGGATCCGA CCCTCCGGCA ATGGATGnifLA gene deletion
BBP1149GACAGGAT CCATATGAAT GCAACCATCC CTCAGCGCT CnifA gene cloning
BBP1150GACATCTA GACTAGATCT TGCGCATGTG GATGTTGAGnifA gene cloning
BBP2099NNNGATAT CGGGCATTCC GCCCGACCTG GTGCTGnifL gene segment deletion 1
BBP2100NNNGATAT CGACGACGAT GCCCTGGGCC AGCAACTGnifL gene segment deletion 1
BBP2101NNNGATAT CCCGGAGAAG GCGCTGCCCT GnifL gene segment deletion 2
BBP2102NNNGATAT CCATCAGCAC CCGCGTGGAG AACnifL gene segment deletion 2
BBP1674NNNTCTAG AGGAAGCTAT CCGACGAGGA CAGCCGAGscrX gene and flanking region cloning
BBP1675NNNGAATT CCTGAGCGCA GAATTTAGAT ATTGATACTC ATAGTCscrX gene and flanking region cloning
BBP1710NNNATGCA TATGGACTTC CTATTGTTGA CATTATTGGT GGscrX gene deletion
BBP1711NNNATGCA TGACAGAATT CAGATCTCAC GCCATAAGCT GTTAGCATTT TTCTTGscrX gene deletion
BBP1669NNNTCTAG AGGATCCCAT ATGCATACCA TGATTACGGA TTCACTGGCC GTCGlacZ gene cloning
BBP1671NNNAAGCT TGGATCCTAT TTTTGACAC CAGACCAACT GGTAATGGTA GlacZ gene cloning
BBP1967NNNAAGCT TCGAAGACAT GGCACTCCGA GGCGTTGGCC AGACamtB gene and flanking region cloning
BBP1968NNNTCTAG AGATAGATTC CCTGCCAGGT CCCCAGamtB gene and flanking region cloning
BBP1969NNNAGATC TGGTTACAAC CTCTGAGTGT CGGGAGamtB gene deletion
BBP1970NNNAGATC TCAGCGTCAT TGATTATTCT CCTGGGGCGamtB gene deletion
BBP982GAAGGGCA GCAGCAGGTA GAGGureABC gene deletion confirmation
BBP983CAGCAGTT CGCGAAGACT GTCGAAGureABC gene deletion confirmation
BBP1836CTCAACGT TCGCCAGGTA TATGCCGAAC TCscrX gene deletion confirmation
BBP1837CACATAGG ATGAAACGTC ACCGAGCTTG TTCGscrX gene deletion confirmation
BBP950GAGCACAC CCATCACGGT CAGAGnifLA gene deletion confirmation
BBP1322GATCTCCA TCGACTCGAT CTTGTCCAGG GTGAACnifLA gene deletion confirmation
BBP2006CACGTGCC AGGAATTCCT CCATGamtB gene deletion confirmation
BBP2007CTGTGGAC GATGGCCAGG GACATGGATCamtB gene deletion confirmation
  • a Specific restriction enzyme sites added to primers are underlined for clarity.