TABLE 1

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Strains
Escherichia coli
    DH5αsupE44 ΔlacU169(ϕ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA127
    BL21For protein expressionNovagen
Xanthomonas oryzae pv. oryzae
    PXO99AWild-type strain, Philippine race 6Lab collection
    ΔPXO_00049 mutantPXO_00049 gene deletion mutant derived from PXO99A, GmrThis study
    ΔPXO_02374 mutantPXO_02374 gene deletion mutant derived from PXO99A, GmrThis study
    ΔPXO_02715 mutantPXO_02715 gene deletion mutant derived from PXO99A, Gmr11
Plasmids
    pColdSUMOExpression vector to generate an N-terminal SUMO-His6 tag, AprHaigene
    pK18mobsacBSuicidal vector carrying sacB gene for mutagenesis, Kmr29
    pBBR1MCS-4Broad-host-range expression vector, Apr30
    pMD18-TCloning vector, AprTaKaRa
    pBlueGFP1Plasmid containing the GFP coding sequence, Apr55
    pMDGFPpMD18-T containing the GFP coding sequence, AprThis study
    pBBGpBBR1MCS-4 containing the GFP coding sequence, AprThis study
    pC49pColdSUMO containing the PXO_00049 coding sequence, AprThis study
    pC2374pColdSUMO containing the PXO_02374 coding sequence, AprThis study
    pC49R141DpColdSUMO containing the point mutant of R141D in PXO_00049 sequence, AprThis study
    pC2374R10DpColdSUMO containing the point mutant of R10D in PXO_02374 sequence, AprThis study
    pK49GpK18mobSacB with PXO_00049 inserted by Gm resistance gene, Kmr GmrThis study
    pK2374GpK18mobSacB with PXO_02374 inserted by Gm resistance gene, Kmr GmrThis study
    pB49pBBR1MCS-4 carrying the PXO_00049 coding sequence, AprThis study
    pB2374pBBR1MCS-4 carrying the PXO_02374 coding sequence, AprThis study
    pB49 R141DpBBR1MCS-4 carrying the PXO_00049 coding sequence, AprThis study
    pB2374 R10DpBBR1MCS-4 carrying the PXO_02374 coding sequence, AprThis study
    pBBG49pBBG carrying the PXO_00049 coding sequence, AprThis study
    pBBG2374pBBG carrying the PXO_02374 coding sequence, AprThis study
    pBBG2715pBBG carrying the PXO_02715 coding sequence, AprThis study