TABLE 2

Virucidal efficacy of silver dihydrogen citrate against FCV on glass measured by plaque assay and qRT-PCR

Contact time (min)Reductiona
Plaque assay (log10 PFU)qRT-PCR (log10 copies)
With 5% serumbControl without 5% serumcWith 5% serumbControl without 5% serumc
11.17 ± 0.30 AA0.77 ± 0.41 AA0.12 ± 0.25 AA−0.85 ± 0.09 AB
53.71 ± 0.35 BA2.49 ± 0.08 BB0.53 ± 0.07 ABA−0.58 ± 0.26 AB
103.84 ± 0.45 B>3.46 A0.45 ± 0.06 BA−0.66 ± 0.10 AB
30>4.66 C>3.46 A1.01 ± 0.28 CA−0.45 ± 0.38 AB
  • a The data are expressed as means ± SDs from 9 replicates in 3 independent experiments. Values with different nonsuperscript letters in the same column are significantly different (P < 0.05), whereas values with different superscript letters in the same row for each detection method are significantly different.

  • b FCV stocks concentrated via ultrafiltration were diluted in complete Dulbecco's modified Eagle's medium with 5% low-endotoxin (<10 EU/ml) fetal bovine serum. Recovered control was 5.68 log10 ± 0.24 log10 PFU.

  • c FCV stocks concentrated via ultrafiltration were diluted in 1× phosphate-buffered saline. Recovered control was 4.50 log10 ± 0.04 log10 PFU.