TABLE 1

Virucidal efficacy of silver dihydrogen citrate against FCV in suspension measured by plaque assay and qRT-PCR

Contact time (min)Reductiona
Plaque assay (log10 PFU)qRT-PCR (log10 copies)
With 5% serumbControl without 5% serumcWith 5% serumbControl without 5% serumc
14.29 ± 0.12 AA4.51 ± 0.09 AB1.93 ± 0.07 AA1.84 ± 0.19 AA
54.37 ± 0.06 ABA4.69 ± 0.28 AB1.85 ± 0.05 AA1.72 ± 0.17 AB
104.41 ± 0.08 BA4.67 ± 0.01 AB1.89 ± 0.04 AA1.84 ± 0.10 AA
304.65 ± 0.11 CA4.65 ± 0.05 AA1.88 ± 0.07 AA1.78 ± 0.08 AA
  • a The data are expressed as means ± standard deviations (SDs) from 9 replicates in 3 independent experiments. Values with different nonsuperscript letters in the same column are significantly different (P < 0.05), whereas values with different superscript letters in the same row for each detection method are significantly different.

  • b FCV stocks concentrated via ultrafiltration were diluted in complete Dulbecco's modified Eagle's medium with 5% low-endotoxin (<10 endotoxin units [EU]/ml) fetal bovine serum.

  • c FCV stocks concentrated via ultrafiltration were diluted in 1× phosphate-buffered saline.