TABLE 3

Virucidal efficacy of steam vapor with TANCS against FCV on glass measured by plaque assay and qRT-PCR

Contact time (s)Reductiona
Plaque assay (log10 PFU)qRT-PCR (log10 copies)
With 5% serumbControl without 5% serumcWith 5% serumbControl without 5% serumc
10>4.93 A>4.11 A2.31 ± 0.25 AA0.70 ± 0.32 AB
30>4.93 A>4.11 A1.92 ± 0.21 AA0.20 ± 0.10 AB
60>4.93 A>4.11 A1.94 ± 0.07 AA0.30 ± 0.38 ABB
90>4.93 A>4.11 A1.93 ± 0.32 AA1.03 ± 0.36 BB
  • a The data are expressed as means ± SDs from 9 replicates in 3 independent experiments. Values with different nonsuperscript letters in the same column are significantly different (P < 0.05), whereas values with different superscript letters in the same row for each detection method are significantly different.

  • b FCV stocks concentrated via ultrafiltration were diluted in complete Dulbecco's modified Eagle's medium with 5% low-endotoxin (<10 EU/ml) fetal bovine serum. Recovered control was 5.99 log10 ± 0.20 log10 PFU.

  • c FCV stocks concentrated via ultrafiltration were diluted in 1× phosphate-buffered saline. Recovered control was 5.17 log10 ± 0.16 log10 PFU.