TABLE 1

Primers used for qPCR analysisa

Primer nameSequence (5′ to 3′)Target (reference)Product size (bp)Annealing temp (°C)
LG1_F1TAYMGNTAYGTNGAYTGGActinorhodopsin (15)30046.6
LG1_F2MGNTAYATHGAYTGGYT
LG1_RATNGGRTANACNCCCCA
SARPR_125FTHGGWGGATAYTTAGGWGAAGCSAR11 Proteorhodopsin (8)20054
SARPR_203RACCTACTGTAACRATCATTCTYA
BACT1369FCGGTGAATACGTTCYCGG16S rRNA (79)300–35058
PROK1541RAAGGAGGTGATCCRGCCGCA
  • a Annealing temperatures for rhodopsin primers were optimized using genomic DNA from R. lacicola (for actinorhodopsin [75]) and a cloned amplicon from the Chesapeake Bay (this work), respectively. 16S primers were tested using R. lacicola. Standard curves for these genes used the same templates.