TABLE 1

Bacterial strains, plasmids, and nematodes used in this study

Bacterial strain, plasmid, or nematodeCharacteristicsaReference or source
Strains
    Escherichia coli
        DH5αsupE44 lacU169 (ϕ80dlacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA146
        OP50Strain used to make lawn to feed C. elegans54
    Bacillus thuringiensis
        BMB171Acrystalliferous mutant20
        C15Wild-type strain harboring Cry5B-like N terminus, a nematicidal strain isolated in China from a soil sampleNot published, stored in authors' lab
        YBT-1518Wild-type nematicidal strain expressing Cry5Ba crystal protein, isolated in China from a soil sample44
Plasmids
    pHT304E. coil-to-Bt shuttle vector; Ampr, Ermr, 6.6 kb45
    pBMB1771Derivative of pHT304, containing a 4-kb genome fragment of C15, which harbored Cry5B-like N-terminal gene fragment under the control of promoter and terminator of Cry5B-like N-terminal gene; Ampr, ErmrThis work
    pBMB1772Derivative of pHT304, containing a 4.1-kb genome fragment of YBY-1518, which harbored Cry5Ba-N terminal gene fragment under the control of promoter and terminator of Cry5Ba; Ampr, ErmrThis work
    pBMB1773Derivative of pHT304, containing the Cry5B-like N terminus from C15 and the C-terminal domain of Cry5Ba from YBT-1518 in frame under the control of promoter of Cry5B-like N terminus and terminator of Cry5Ba; Ampr, ErmrThis work
    pBMB1774Derivative of pHT304, containing a 3.5-kb fragment of Cry5Ba C terminus with its promoter, and terminator of Cry5Ba; Ampr, ErmrThis work
Nematodes (C. elegans)
    N2Wild-type strainCGC
    FT63GFP-labeled mutant strain expressed (dlg::gfp) in epithelial cell junction34
    bre-2Cry5B-resistant mutant strain lacked the glycolipid receptor for Cry5B50
  • a Ampr, ampicillin resistant; Ermr, erythromycin resistant.