TABLE 4

Profiles of phytosterol transformation by 9-OHAD-producing strains for 120 h

Strain and concn of substratea (g liter−1)Major productConcnb (g liter−1)Molar yieldc (%)Yield ratio of 9-OHAD (%)
IV
    29-OHAD1.10 ± 0.0574.8 ± 3.195.7 ± 0.2
AD0.05 ± 0.023.4 ± 1.0
    209-OHAD10.04 ± 0.2268.3 ± 1.589.0 ± 0.5
AD1.24 ± 0.118.4 ± 0.5
V
    29-OHAD1.32 ± 0.0390.2 ± 1.997.6 ± 0.3
AD0.03 ± 0.012.2 ± 0.3
    209-OHAD11.71 ± 0.1579.8 ± 1.094.2 ± 0.4
AD0.71 ± 0.104.9 ± 0.6
  • a The conversion of 2 g liter−1 phytosterols was performed in shake flasks using growing cells, and the conversion of 20 g liter−1 phytosterols was performed in a 7-liter bioreactor using resting cells. Data are shown as the averages from triplicate experiments.

  • b Amounts of major metabolites and their molar yields were determined from three independent transformations and are expressed as the actual range from the least to the topmost.

  • c The yield ratio of 9-OHAD was obtained by calculating the molar yield of 9-OHAD among all the products.