TABLE 2

Lachno3 and Lachno12 marker primer and probe sequences

MarkerSequence
Forward primerProbeaReverse primer
Lachno35′-CAACGCGAAGAACCTTACCAAA-3′FAM-5′-CTCTGACCGGTCTTTAATCGGA-3′-MGB5′-CCCAGAGTGCCCACCTTAAAT-3′
Lachno125′-ATCTTGACATCCCTCTGACCGGGA-3′FAM-5′-CGTCCCTTTCCTTCGGGACAGG-3′-MGB5′-CTCAGAGTGCCCACCACTACGT-3′
  • a FAM, 6-carboxyfluorescein; MGB, TaqMan minor groove binder.