TABLE 2

Endpoint metabolite concentrations after 72 h of static-flask growth

StrainNet product titer (mM)a
ButanolAcetoneEthanolAcetateButyrateGlucoseb
WT135.0 ± 27.070.8 ± 4.819.2 ± 5.51.2 ± 6.66.2 ± 5.6276.9 ± 52.1
824(p94MCS)150.4 ± 16.173.9 ± 4.929.6 ± 4.9−1.3 ± 1.93.6 ± 4.3311.8 ± 24.2
824(p94_solB)19.8 ± 6.26.6 ± 2.53.7 ± 1.214.8 ± 3.250.9 ± 3.9105.2 ± 5.3
824 ΔsolB5.5 ± 1.80.0 ± 0.02.7 ± 0.612.5 ± 1.665.7 ± 1.885.8 ± 10.4
  • a Values are averages (n = 3) ± SD and are normalized to the level at the first time point. Metabolite profiles are shown in Fig. 3.

  • b Total glucose consumption after 72 h of static growth.