TABLE 1

Strains generated by experimental evolution, by P1 construction, or by recombineering

StrainPopulation isolateDescription or genotypeReferencea
W3110Escherichia coli K-12
JLSH001B11-1W3110 evolved at pH 9.25This study
JLSH002B11-2W3110 evolved at pH 9.25This study
JLSH003F7-1W3110 evolved at pH 9.25This study
JLSH004F7-2W3110 evolved at pH 9.25This study
JLSH005D9-1W3110 evolved at pH 9.25This study
JLSH006D9-2W3110 evolved at pH 9.25This study
JLSH007G9-1W3110 evolved at pH 9.25This study
JLSH008G9-2W3110 evolved at pH 9.25This study
JLS1307W3110 ΔrpoS::kanRThis study
JLS1622W3110 ΔtorI::kanRThis study
JLS1628W3110 ΔtorR::kanRThis study
JLS1714JLSH007 (G9-1) ΔtorR::kanRThis study
JLS1728W3110 ΔtorS::kanRThis study
JLS1729JLSH007 (G9-1) ΔtorS::kanRThis study
JLS1775JLSH007 (G9-1) ΔtorCADThis study
SG30013DJ480 (rpoS750::lacZ translational fusion)46
INH5SG30013 ΔiraM::tet ΔiraD::zeo ΔiraP::kanThis study
INH12W3110 ΔphoB::kanRThis study
INH14W3110 ΔydcI::kanRThis study
INH16W3110 ΔpcnB::kanRThis study
NM376nlpD-rpoS promoter region −775 to +10 fused to lacZThis study
NM378nlpD-rpoS promoter region −620 to +10 fused to lacZThis study
BA943Cm-Cp17-C125T mutant rpoS477-lacZ34
BA938Cm-Cp17-rpoS477 lacZ34
SG30018SG30013; ΔrssB::tet52
BA926Cm-Cp17-lac-lacZ34
XTL241W3110 galKtyr145UAG ΔlacU169cI857 Δ(cro-bioA)] gam<>cat sacB53
  • a Strains indicated as created for this study are described further in Materials and Methods or elsewhere in the text (for evolved strains).