TABLE 1

Kinetic parameters of wild-type PlcP7211, wild-type PlcP193, and mutant PlcP193 enzymes following the hydrolysis of p-nitrophenylphosphorylcholine (NPPC)a

Proteinkcat (s−1)Km (μM)kcat/Km (s−1 mM−1)
PlcP721141.8 ± 6.3275.6 ± 5.7151.7 ± 5.9
PlcP19338.6 ± 5.2234.2 ± 6.3164.8 ± 5.6
H82R25.5 ± 3.8182.5 ± 5.3140.0 ± 3.9
H82N18.6 ± 4.2154.8 ± 4.8120.1 ± 4.5
H82A0.12 ± 0.117.2 ± 2.37.0 ± 2.5
  • a Values are means ± standard deviations from three independent experiments.