TABLE 1

LT50 values for B. bassiana wild-type, ΔBbgas3 mutant, and complemented (ΔBbgas3::Bbgas3) strains in topical and intrahemocoel injection bioassays using G. mellonella larvae as the host

PDA pH valueaB. bassiana strainLT50 (days)
Topical infectionInjection
5.6WT5.09 ± 0.044.62 ± 0.02
ΔBbgas3 mutant5.46 ± 0.243.87 ± 0.17
ΔBbgas3::Bbgas3 complement5.47 ± 0.374.53 ± 0.40
7WT5.31 ± 0.203.36 ± 0.03
ΔBbgas3 mutant4.89 ± 0.163.33 ± 0.08
ΔBbgas3::Bbgas3 complement4.87 ± 0.193.30 ± 0.03
10WT5.65 ± 0.183.34 ± 0.09
ΔBbgas3 mutant6.94 ± 0.355.71 ± 0.11
ΔBbgas3::Bbgas3 complement5.84 ± 0.023.40 ± 0.02
  • a Fungal conidia derived from growth on PDA were amended to the indicated pH values and used in the bioassays.