TABLE 1

Formation of sublethally injured cells after treatment with different concentrations of NEO water

NEO water concn (%)Free chlorine concn (ppm)Y. enterocoliticaE. coli O157: H7S. Enteritidis
Count (log CFU/ml) ona:%bCount (log CFU/ml) ona:%Count (log CFU/ml) ona:%
TSAYECINTSAYESMACTSAYEXLD
008.42 ± 0.10 A8.40 ± 0.05 ANA8.61 ± 0.02 A8.58 ± 0.01 ANA8.08 ± 0.16 A7.82 ± 0.12 ANA
31.88.33 ± 0.08 A8.27 ± 0.08 ANA8.55 ± 0.03 A8.54 ± 0.05 ANA8.01 ± 0.09 A7.60 ± 0.07 A*52.00
63.58.08 ± 0.10 B7.30 ± 0.13 B*38.127.91 ± 0.04 B7.85 ± 0.06 BNA7.54 ± 0.11 B6.71 ± 0.08 B*24.57
105.97.13 ± 0.12 C6.14 ± 0.12 C*4.5666.71 ± 0.03 C6.69 ± 0.04 CNA5.78 ± 0.09 C5.48 ± 0.07 C*0.250
158.95.91 ± 0.04 D5.41 ± 0.05 D*0.2114.89 ± 0.16 D4.78 ± 0.03 DNA3.61 ± 0.21 D3.04 ± 0.32 DNA
2514.83.90 ± 0.12 E2.70 ± 0.35 E*0.002NDNDNANDNDNA
  • a Different letters within each column represent significant differences between treatments with different NEO water concentrations (P < 0.05). *, a significant difference existed (P < 0.05) between the counts obtained from TSAYE and the counts obtained from the corresponding selective agar by using a two-tailed Student's t test. ND, the surviving cell numbers were below the limit of enumeration.

  • b Percentage of sublethally injured cells. Percentages were calculated only when significant differences in cell counts between the selective agar and the nonselective agar were observed. NA, not applicable.