TABLE 1

Bacterial strains and plasmids used in this study

Bacterial strain or plasmidCharacteristic(s) or genotypeaReference or source
Strains
    S. enterica serovar Kentucky (S.KTn7lux)Wild type::luxCDAB26
    E. coli DH5αContains lux gene26
    S.KΔfliCMutation in alternative flagellin subunit, Aps, CmsThis study
    S.KΔfljBMutation in alternative flagellin subunit, Aps, CmsThis study
    S.KΔmotAMutation in motion gene, Aps, GmsThis study
    S.KΔflgKMutation in hook-associated protein, Aps, GmsThis study
    S.KΔfliCΔfljBDouble mutation in both flagellin subunits, Aps, GmsThis study
    S.KΔflhB-tTransposon mutation in flagellar export apparatus, Gmr13
    S.KΔflgA-tTransposon mutation in basal body p-ring protein, Gmr13
    S.KΔflgK-tTransposon mutation in hook-associated protein, Gmr13
    S.KΔflgJ-tTransposon mutation in rod assembly protein, Gmr13
    S.KΔflgC-tTransposon mutation in basal body rod protein, Gmr13
    S.KΔmotACComplemented strain of S.KΔmotA mutant, AprThis study
    S.KΔflgKCComplemented strain of S.KΔflgK mutant, AprThis study
    S.KΔfliCΔfljBCComplemented strain of S.KΔfliCΔfljB mutant, AprThis study
    E. coli K-12 (BW25141/pKD3)lacIq rrnBT14 ΔlacZWJ16 ΔphoBR580 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 galU95 endABT333 uidA(ΔMluI) pir+ recA112
    E. coli K-12 (BW25141/pKD4)lacIq rrnBT14 ΔlacZWJ16 ΔphoBR580 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 galU95 endABT333 uidA(ΔMluI) pir+ recA112
    E. coli DH5α/pCP20Δ(argF-lac)169 ϕ80dlacZ58 glnV44 λrfbC1 gyrA96 recA1 spoT1 thi-1 hsdR1712
    E. coli K-12 (BW25113)/pKD46lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD7812
Plasmids
    pCP20Ts-rep [c857] (lambda)ts bla(apr) cat (FLP)12
    pKD46repA101(ts) araBp-gam-bet-exo oriR101 bla(apr)12
    pKD3oriR6Kγ bla(apr) rgnB(Ter) cat12
    pKD4oriR6Kγ bla(apr) rgnB(Ter) kan12
    pBEN276mTn7::MC apr luxCDABE26
    pBBR1MCS-4Expression vector; apr28
    pBBR1MCS-4 flgKpBBR1MCS-4 expressing wild-type flgKThis study
    pBBR1MCS-4 fliCpBBR1MCS-4 expressing wild-type fliCThis study
    pBBR1MCS-4 motApBBR1MCS-4 expressing wild-type motAThis study
  • a Aps, ampicillin susceptibility; Cms, chloramphenicol susceptibility; Gms, gentamicin susceptibility; Gmr, gentamicin resistance; Apr, ampicillin resistance.