TABLE 3

Primers that have been applied for junction confirmation of constructed mutants

GeneUpstream chloramphenicol-gene junctionDownstream chloramphenicol-gene junctionUpstream-downstream genes
flgK (A)C2, flgL (A1)A2: C1, flgJ (A2)flgL, flgJ (A3)
fliC (B)C2, sekA-A1494 (B1)C1, sekA-A1497 (B2)sekA-A1494, sekA-A1497 (B3)
motA (C)C2, motB (C1)C1, flhC (C2)motB, flhC (C3)
fljB (D)C2, sekA-A2274 (D1)C1, sekA-A2276 (D2)sekA-A2274, sekA-A2276 (D3)