TABLE 1

Strains and plasmids used in this study

Strain or plasmidRelevant genotype and/or descriptionaReference or source
Strains
    R. palustris
        CGA009Wild-type strain27
        CGA4000CGA009 derivative; ΔuppE (ΔRPA2750) mutantThis study
        CGA4022CGA009 derivative; ΔuppC (ΔRPA4833) mutantThis study
    E. coli
        S17-1thi pro hsdR hsdM+ recA; chromosomal insertion of RP4-2 (Tc::Mu Km::Tn7)51
        DH5-αF λ recA1 Δ(lacZYA-argF)U169 hsdR17 thi-1 gyrA96 supE44 endA1 relA1 φ80lacZΔM15Thermo Fisher Scientific
Plasmids
    pJQ200KSGmr; sacB; R. palustris suicide vector50
    pJQ-RPA2750Gmr; sacB; derived from pJQ200KS; deletion vector for uppE (RPA2750)59
    pJQ-RPA4833Gmr; sacB; derived from pJQ200KS; deletion vector for uppC (RPA4833)This study
    pGEMHigh-copy-no. cloning vector for insertion of PCR productsPromega
    pBBPgdhGmr; broad-host-range cloning vector with constitutive R. palustris gapdh promoter53
    pBBP-RPA2750Gmr; derived from pBBPgdh; complementation vector for ΔuppE (ΔRPA2750)This study
    pBBP-RPA4833Gmr; derived from pBBPgdh; complementation vector for ΔuppC (ΔRPA4833)This study
  • a Gmr, gentamicin resistant.