TABLE 1

Properties of identified ORFs and protein coding sequences (wild type) for six novel metagenome esterases

ORFLength (bp)GC content (%)Protein sequence
Size (amino acids)Estimated molecular mass (kDa)Predicted isoelectric pointPredicted signal peptideaMaximal amino acid sequence identity (GenBank accession no.)b
EstB31,22764.940844.546.28No63% vs serine hydrolase from Tardiphaga sp. Vaf07 (WP_068735481)
EstB111,15262.238340.315.33No55% vs 1,4-butanediol diacrylate esterase from Thermorudis peleae (WP_038038941)
EstC591863.930532.766.50N-terminal (∼34 amino acids)61% vs esterase/lipase from Caulobacter vibrioides (WP_004617716)
EstC71,15563.638340.205.43No53% vs 1,4-butanediol diacrylate esterase from Thermorudis peleae (WP_038038941)
EstC988865.129530.827.78No56% vs α/β-hydrolase from Acidocella sp. MX-AZ02 (WP_008494015)
EstG496040.331935.805.61N-terminal (∼19 amino acids)37% vs hypothetical protein ACD_45C00716G0002 from uncultured bacterium (EKD72285)
  • a Signal peptide prediction was performed with SignalP 4.1, with a cutoff value for discrimination scores of 0.500.

  • b BLASTP analysis against the GenBank database of full-length nonredundant protein sequences (23 August 2016 version) yielded the maximal identity to the closest annotated hit.