TABLE 2

Primers used in this study

PrimerSequence (5′→3′)aDescription (restriction site)
UuppE-XbaICGCGGTGGCGGCCGCTCTAGAAAGCATCACGGATCTGTTCGTCTGuppE (RPA2750) upstream flanking region (XbaI)
UuppE-delRGCGAACGCCTCAGTAGGTACCGCTGATCGGCTCCATCTGTTCATGuppE (RPA2750) upstream in-frame deletion reverse
DuppE-delFATGGAGCCGATCAGCGGTACCTACTGAGGCGTTCGCTCTTCAACAuppE (RPA2750) downstream in-frame deletion forward
DuppE-BamHITTCCTGCAGCCCGGGGGATCCAGAGCAACAACAACCAAAGGGAGCuppE (RPA2750) downstream flanking region (BamHI)
uppE-compF-BamHICTGATCTAGAAGCACGGTGGATATGGATTCCuppE (RPA2750) complementation forward (BamHI)
uppE-compR-XbaIGACTGGATCCCCGGACGACAAAGTCGTGuppE (RPA2750) complementation reverse (XbaI)
UuppC-XbaIGACTTCTAGAACCCATTTCGTGAGTGGCAACCuppC (RPA4833) upstream flanking region (XbaI)
UuppC-delRAGAACCAGCGTTCGATGATCATCGATACTTGAAACGCGCuppC (RPA4833) upstream in-frame deletion reverse
DuppC-delFGATGATCATCGAACGCTGGTTCTGAACCGGuppC (RPA4833) downstream in-frame deletion forward
DuppC-BamHIGACTTCTAGACGGTTTCGAACTCGGGGGTTATuppC (RPA4833) downstream flanking region (BamHI)
uppC-compF-BamHIACAGCGGGATCCCGTGGCGAGGGATGGCuppC (RPA4833) complementation forward (BamHI)
uppC-compR-XbaIACAGCGTCTAGATCAGAACCAGCGTTCGCCGAuppC (RPA4833) complementation reverse (XbaI)
  • a Restriction sites are underlined.