TABLE 1

Results from optimization studies showing the effect of sample size, volume, and temperature of BPW and temperature of incubation on growth of Salmonella

Sample sizebVol of BPW (ml)Temp of BPW (°C)Temp of incubator (°C)Temp (°C) ata:Salmonella growth (log CFU/ml)
0 min60 min120 min180 min
10 by 25 cm21004541.523.0 ± 0.929.9 ± 0.735.7 ± 0.338.8 ± 0.21.7 ± 0.2
10 by 25 cm2100373721.2 ± 1.027.3 ± 3.030.8 ± 2.032.6 ± 0.50.7 ± 0.1
5 by 25 cm21004541.526.335.238.740.12.0 ± 0.2
1 by 25 cm21004541.534.537.739.440.22.2 ± 0.1
1 by 25 g30373722.0 ± 0.0 34.0 ± 0.91.2 ± 0.2
  • a Data are shown as the means and 95% confidence intervals (CIs); if no CI is shown, data were based on a single experiment.

  • b Sample size is presented as the number of samples by the mass. A 25-cm2 sample weighed between approximately 11 and 17 g.