TABLE 2

Bacterial strains and plasmids used in this study

Strain or plasmidGenotypeReference or source
Escherichia coli strains
    XL-1 BluerecA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)]Stratagene
    SM-10Conjugation donor; F thi-1 thr-1 leuB6 recA tonA21 lacY1 supE44 (Muc+) λ [RP4-2(Tc::Mu)] Kmr Tra+33
Listeria monocytogenes strains
    10403SWT, StreprD. A. Portnoy, lab stock
    A118 cured10403S ΔcomK phage23
    Δmonocin mutant10403S ΔcomK phage ΔLMRG_2362-2337This study
    ΔftbQR mutant10403S ΔcomK phage ΔLMRG_2377-2378This study
    WT + pPL2 pConst pheS*10403S harboring pPL2 integrative plasmid expressing a mutated B. subtilis pheS, p-Cl-pheS, CmrThis study
    WT + pPL2 pConst GFP10403S harboring pPL2 integrative plasmid expressing GFPThis study
Plasmids
    pPL2E. coli/L. monocytogenes conjugative vector, integrative to tRNA-Arg gene, Cmr18
    pBHE261E. coli/L. monocytogenes conjugative vector, pKSV7 temp-sensitive Ori, Amp−r, Cm+r35
    pLR16-pheS*E. coli/L. monocytogenes conjugative vector, pKSV7 temp-sensitive Ori, p-Cl-pheS, CmrThis study
    pLR16ΔmonocinpLR16 with upstream and downstream homology fragments insertedThis study
    pLR16ΔftbQ-RpLR16 with upstream and downstream homology fragments insertedThis study
  • aAmpr, ampicillin resistance; Kmr, kanamycin resistance; Cmr, chloramphenicol resistance; p-Cl-Phes, p-chloro-phenylalanine sensitivity. −, Gram-negative resistance; +, Gram-positive resistance.