TABLE 1

Strains used

Strain nameGenotypeReference/source or construction
MHM80ATCC 14028 glnA15::FRT-kan-FRTConstructed using Datsenko and Wanner mutagenesis with primers MHM197 and MHM198, confirmed with primers MHM199 and MHM200
MHM85ATCC 14028 glnA15::FRT-kan-FRTS. enterica serovar Typhimurium ATCC 14028 × P22/MHM80
MHM101JS246 glnA15::FRT-kan-FRTJS246 × P22/MHM85
MHM130JS246 glnA15::tnpR-lacZYJS246 glnA15::FRT electroporated with pCE71
MHM84ATCC 14028 argA16::FRT-kan-FRTConstructed using Datsenko and Wanner mutagenesis with primers MHm201 and MHM202, confirmed with primers MHM276 and MHM277
MHM90ATCC 14028 argA16::FRT-kan-FRTS. enterica serovar Typhimurium ATCC 14028 × P22/MHM84
MHM97JS246 argA16::FRT-kan-FRTJS246 × P22/MHM90
MHM129JS246 argA16::tnpR-lacZYJS246 argA16::FRT electroporated with pCE70
MHM63ATCC 14028 proA17::FRT-kan-FRTConstructed using Datsenko and Wanner mutagenesis with primers MHM205 and MHM206, confirmed with primers MHM207 and MHM208
MHM74ATCC 14028 proA17::::FRT-kan-FRTS. enterica serovar Typhimurium ATCC 14028 × P22/MHM63
MHM65ATCC 14028 metA18::FRT-kan-FRTConstructed using Datsenko and Wanner mutagenesis with primers MHM209 and MHM210, confirmed with primers MHM211and MHM212
MHM69ATCC 14028 metA18::FRT-kan-FRTS. enterica serovar Typhimurium ATCC 14028 × P22/MHM65
MHM86JS246 metA18::FRT-kan-FRTJS246 × P22/MHM65
MHM91JS246 metA18:: tnpR-lacZYJS246 metA::FRT electroporated with pCE70
MHM62ATCC 14028 serA19::FRT-kan-FRTConstructed using Datsenko and Wanner mutagenesis with primers MHM213 and MHM214, confirmed with primers MHM215 and MHM216
MHM75ATCC 14028 serA19::FRT-kan-FRTS. enterica serovar Typhimurium ATCC 14028 × P22/MHM62
MHM98JS246 serA19::FRT-kan-FRTJS246 × P22/MHM62
MHM106JS246 serA19::tnpR-lacZYJS246 serA19::FRT electroporated with pCE71
MHM64ATCC 14028 thrC20::FRT-kan-FRTConstructed using Datsenko and Wanner mutagenesis with primers MHM217 and MHM218, confirmed with primers MHM219 and MHM220
MHM70ATCC 14028 thrC20::FRT-kan-FRTS. enterica serovar Typhimurium ATCC 14028 × P22/MHM64
MHM87JS246 thrC20::FRT-kan-FRTJS246 × P22/MHM64
MHM92JS246 thrC20::tnpR-lacZYJS246 thrC20::FRT electroporated with pCE70
MHM66ATCC 14028 trpC21::FRT-kan-FRTConstructed using Datsenko and Wanner mutagenesis with primers MHM221 and MHM222, confirmed with primers MHM223 and MHM224
MHM73ATCC 14028 trpC21::FRT-kan-FRTS. enterica serovar Typhimurium ATCC 14028 × P22/MHM66
MHM88JS246 trpC21::FRT-kan-FRTJS246 × P22/MHM66
MHM95JS246 trpC21::tnpR-lacZYJS246 thrC21::FRT electroporated with pCE70
MHM72ATCC 14028 ilvD22::FRT-kan-FRTConstructed using Datsenko and Wanner mutagenesis with primers MHM225 and MHM226, confirmed with primers MHM270 and MHM270
MHM77ATCC 14028 ilvD22::FRT-kan-FRTS. enterica serovar Typhimurium ATCC 14028 × P22/MHM72
MHM96JS246 ilvD22::FRT-kan-FRTJS246 × P22/MHM72
MHM132JS246 ilvD22::tnpR-lacZYJS246 ilvD22::FRT electroporated with pCE70
MHM83ATCC 14028 purH23::FRT-kan-FRTConstructed using Datsenko and Wanner mutagenesis with primers MHM233 and MHM234, confirmed with primers MHM270 and MHM271
MHM89ATCC 14028 purH23::FRT-kan-FRTS. enterica serovar Typhimurium ATCC 14028 × P22/MHM83
MHM67ATCC 14028 pyrB24::FRT-kan-FRTConstructed using Datsenko and Wanner mutagenesis with primers MHM237 and MHM238, confirmed with primers MHM239 and MHM240
MHM68ATCC 14028 pyrB24::FRT-kan-FRTS. enterica serovar Typhimurium ATCC 14028 × P22/MHM67
MHM94ATCC 14028 gltB25::FRT-kan-FRTConstructed using Datsenko and Wanner mutagenesis with primers MHM282 and MHM283, confirmed with primers MHM284, MHM285 and MHM286
MHM103ATCC 14028 gltB25::FRT-kan-FRTS. enterica serovar Typhimurium ATCC 14028 × P22/MHM94
MHM126JS246 gltB25::FRT-kan-FRTJS246 × P22/MHM94
MHM131JS246 gltB25::tnpR-lacZYJS246 gltB25::FRT electroporated with pCE70
MM_015_H02 (MHM110)ATCC 14028 rfbA26::FRT-kan-FRT38
MM_015_G02 (MHM111)ATCC 14028 rfaI27::FRT-kan-FRT38
MM_011_G12 (MHM138)ATCC 14028 rfbN28::FRT-kan-FRT38
MM_011_D12 (MHM139)ATCC 14028 rfbP30::FRT-kan-FRT38
MHM42ATCC 14028 (invH-avrA)::FRTS. enterica serovar Typhimurium ΔSPI-1 (23)
MHM43ATCC 14028 (sugR-mtgC)::FRTS. enterica serovar Typhimurium ΔSPI-3 (23)
MHM48ATCC 14028 (ssrB-ssaU)::FRTS. enterica serovar Typhimurium ΔSPI-2 (23)
MHM53ATCC 14028 phoN::FRT-kan-FR23