TABLE 2

The effect of pykA inactivation on utilization of glucose for n-butanol productiona

StrainOD600Glucose consumed (g/liter)Level of product (g/liter)% yield (g/g)
Pyruvaten-Butanol
EB2161.44 ± 0.028.09 ± 0.401.28 ± 0.041.38 ± 0.1617
EB216-Tn5-599#1.83 ± 0.0410.88 ± 0.210.59 ± 0.072.52 ± 0.1823
EB216-ΔpykA1.74 ± 0.0210.49 ± 0.150.43 ± 0.092.37 ± 0.1023
  • a The strains were grown in static 15-ml sealed BD tubes for determination of n-butanol production at 37°C for 48 h. EB216 is the control strain. The pykA gene was inactivated by insertion of a Tn5 transposon in strain EB216-Tn5-599# or by deletion in strain EB216-ΔpykA. Three repeats were performed, and the error data represent standard deviations.