TABLE 1

Bacterial strains and plasmids used in this study

Bacterial strain or plasmidCharacteristicsaSource or reference
Strains
    Burkholderia symbiont
        RPE75Burkholderia symbiont (RPE64); Rifr46
RPE75 ΔphaP1; RifrThis study
RPE75 ΔphaP2; RifrThis study
RPE75 ΔphaP3; RifrThis study
RPE75 ΔphaP4; RifrThis study
RPE75 ΔphaR; RifrThis study
RPE75 ΔphaC; Rifr25
RPE75 ΔphaR/phaR; RifrThis study
    Escherichia coli
        DH5αF Φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK+) phoA supE44 λ thi-1 gyrA96 relA1Invitrogen
Plasmids
    pK18mobsacBpMB1ori allelic exchange vector containing oriT; Kmr47
    pBBR122Broad-host-range vector; Cmr Kmr38
  • a Rifr, rifampin resistance; Kmr, kanamycin resistance; Cmr, chloramphenicol resistance.