TABLE 6

Assay of intracellular concentrations of pyruvate, ATP, and NADHa

StrainIntracellular level
Pyruvate (μmol/mg protein)ATP (nmol/mg protein)NADH (pmol/mg protein)
EB2160.33 ± 0.043.37 ± 0.483,690 ± 355
EB216-ΔpykF0.31 ± 0.022.33 ± 0.223,203 ± 277
EB216-ΔpykA0.39 ± 0.018.43 ± 0.472,903 ± 193
  • a mg protein, milligrams of crude protein extract. The strains were grown in static 15-ml sealed BD tubes at 37°C. Three repeats were performed, and the error data represent standard deviations.