TABLE 2

Sequences of primers used in this study

PrimerSequence (5′–3′)
PRMC2-NcoI-FCATTCTCTGGTATTTGGACTCCTG
PRMC2-SapI-RAGTCAGTGAGCGAGGAAGCGTAAGAGCGCCCAATAC
PRMC2-SapI-FGTATTGGGCGCTCTTACGCTTCCTCGCTCACTGACT
PRMC2-EcoRI-RAGCTTGATGGTACCGTTAACAGA
PpflB-SmaI-FTAGCCCCGGGATATACTCCTAAATTAACTT
PpflB-RTAGTCATTGTAGCATGTTTGTTGTG
sgRNA-SapI-FCACAACAAACATGCTACAATGACTATGAAGAGC CATGTCAGGCTCTTCTGT
sgRNA-SapI-RACTTGCTATTTCTAGCTCTAAAACAGAAGAGCCT GACATGGCTCTTCATAG
sgRNA-FGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAA
sgRNA-RAAAAAAGCACCGACTCGGTGCCACTTTTTCAAG TTGATAACGGACTAGCCTTATTT
sgRNA-slice-BglII-FGAAGATCTTAGCCCCGGGATATACTCC
sgRNA-slice-EcoRI-RCGGAATTCAAAAAAGCACCGACTCGG
sgRNA-cassette-FCGGAATTCATATACTCCTAAATTAACTT
sgRNA-cassette-RCGGAATTCAAAAAAGCACCGACTCGG
dcas9-BglII-FGAAGATCTGGGAGGCCGTTTCATGGATAA
dcas9-SmaI-RTCCCCCGGGTATAAACGCAGAAAGGCCCAC
hla-sgRNA1-oligo1CTAGTTGAATAAATTCTTTATGAAACA
hla-sgRNA1-oligo2AACTGTTTCATAAAGAATTTATTCAAC
hla-sgRNA2-oligo1CTACATTAGCGACAGGATTCATTAATA
hla-sgRNA2-oligo2AACTATTAATGAATCCTGTCGCTAATG
hla-sgRNA3-oligo1CTACGGGTTCCAAGAATCTCTATCATA
hla-sgRNA3-oligo2AACTATGATAGAGATTCTTGGAACCCG
hla-sgRNA4-oligo1CTACTCAGTAACAACAACACTATTGCT
hla-sgRNA4-oligo2AACAGCAATAGTGTTGTTGTTACTGAG
mecA-sgRNA1-oligo1CTAACAACTACAACTATTAAAATAAG
mecA-sgRNA1-oligo2AACCTTATTTTAATAGTTGTAGTTGT
spa-sgRNA1-oligo1CTATCGTGTTGCGCAGCATTTGCAGC
spa-sgRNA1-oligo2AACGCTGCAAATGCTGCGCAACACGA
sarT-sgRNA1-oligo1CTATAACTCCAAATATATTTAGTTTG
sarT-sgRNA1-oligo2AACCAAACTAAATATATTTGGAGTTA
sarT-sgRNA2-oligo1CTATTACGCTTTTAACAATTCTAGAT
sarT-sgRNA2-oligo2AACATCTAGAATTGTTAAAAGCGTAA
ccrAB-sgRNA1-oligo1CTAATAGCCTATGACTTGTTTCATAT
ccrAB-sgRNA1-oligo2AACATATGAAACAAGTCATAGGCTAT
ccrAB-sgRNA2-oligo1CTAGATATGTTGAATATGATGTTTTT
ccrAB-sgRNA2-oligo2AACAAAAACATCATATTCAACATATC
ccrAB-sgRNA3-oligo1CTACGTTTATGCAATCGATGATTGCA
ccrAB-sgRNA3-oligo2AACTGCAATCATCGATTGCATAAACG
ccrAB-sgRNA4-oligo1CTAATATATCTTTAACTTCAAAATGA
ccrAB-sgRNA4-oligo2AACTCATTTTGAAGTTAAAGATATAT
era-sgRNA1-oligo1CTAATATCGTCTATTTGTATAATTCC
era-sgRNA1-oligo2AACGGAATTATACAAATAGACGATAT
era-sgRNA2-oligo1CTACCCTAAAATAACGCTATAAAATG
era-sgRNA2-oligo2AACCATTTTATAGCGTTATTTTAGGG
era-sgRNA3-oligo1CTAACAAATGTTGACTTTCCTACATT
era-sgRNA3-oligo2AACAATGTAGGAAAGTCAACATTTGT
era-sgRNA4-oligo1CTACTGCTTTGATGATAATCCCTTTT
era-sgRNA4-oligo2AACAAAAGGGATTATCATCAAAGCAG
murE-sgRNA-oligo1CTAATACTAGCTACTCTTAATGTGTC
murE-sgRNA-oligo2AACGACACATTAAGAGTAGCTAGTAT
rpsC-sgRNA1oligo1CTAATAATACCAACACGAAGTCCGAT
rpsC-sgRNA-oligo2AACATCGGACTTCGTGTTGGTATTAT
walR-sgRNA-oligo1CTATTAAATTCTAAAATATCAGCAAT
walR-sgRNA-oligo2AACATTGCTGATATTTTAGAATTTAA
RT-pta-FAAAGCGCCAGGTGCTAAATTAC
RT-pta-RCTGGACCAACTGCATCATATCC
RT-hla-FAAAGTAGGCTGGAAAGTGAT
RT-hla-RTAGCGAAGTCTGGTGAAAA
RT-spa-FAAGATGGTAACGGAGTACATGTCG
RT-spa-RCAAGTTCTTGACCAGGTTTGATC
RT-ccrA-FGCACAGTTATTAGAAGAAGATA
RT-ccrA-RGCCATATTGATTGTTGACA
RT-ccrB-FCAATACCACGAATACACTT
RT-ccrB-RCATCACATAATCTTCAATCAC
RT-era2-FTGCTTTAGAAGAAGATGAGCCAGAG
RT-era2-RGTCCGTAATTGTTTGCTTGTTCT
RT-era4-FGTTAGAAAAGCACAACAAGAATC
RT-era4-RTAGCTCGTTCAGCACATATCG
RT-era5-FAAGGTGTTATGACAAGAGATGACG
RT-era5-RAGCGACTTTCATCATATAGTCACC
RT-era6-FGGTGCAAAAGTACCACTTATGGC
RT-era6-RTTCCCATACCTCGCCACTGATT
pRMC2-seq-FCACAGATGCGTAAGGAGA