TABLE 1

S. cerevisiae strains used in this study

StrainGenotypeSource or reference
LRB341MATa ura3-52 leu2 his358
LRB362MATa ura3-52 leu2 his3 yck1-1::ura3 yck2-2ts58
BY4741MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0Saccharomyces Genome Deletion Project
ZLY4260BY4741 yck3Δ::kanMX4Saccharomyces Genome Deletion Project
ZLY962BY4743 hrr25Δ::kanMX4/HRR25Saccharomyces Genome Deletion Project
ZLY4467BY4741 hrr25Δ::kanMX4 [pRS415-HRR25(E52D)]This study
ZLY4501BY4741 hrr25Δ::kanMX4This study
ZLY4043BY4741 haa1Δ::kanMX4Saccharomyces Genome Deletion Project
ZLY4637BY4741 hrr25Δ::kanMX4 haa1::HIS3 [pRS415-HRR25(E52D)]This study
ZLY5057LRB341 haa1::HIS3This study
ZLY5058LRB362 haa1::HIS3This study
ZLY5173BY4741 yck3Δ::kanMX4 haa1::HIS3This study
ZLY5046MATα ura3 leu2 his3 met15 lys2 hrr25Δ::kanMX4 haa1::HIS3This study
ZLY4479MATα ura3 leu2 his3 met15 lys2 hrr25Δ::kanMX4This study
AH109MATa ura3-52 his3-200 trp1-901 leu2-3,112 gal4Δ gal80Δ URA3::MEL1UAS-MEL1TATA-lacZ GAL2UAS-GAL2TATA-ADE2 LYS2::GAL1UAS-GAL1TATA-HIS3Clontech Laboratories, Inc.