TABLE 2

Plasmids used in this study

PlasmidDescriptionaSource
pZL3158pRS416-TPO2-lacZ, expressing TPO2-lacZ reporter gene with 1,926-bp TPO2 promoter sequence fused to lacZ coding sequenceThis study
pZL3164pRS416-YRO2-lacZ, expressing YRO2-lacZ reporter gene with 1,929-bp YRO2 promoter sequence fused to lacZ coding sequenceThis study
pZD359pRS415-HRR25(E52D), expressing missense HRR25 mutant allele substituting for glutamate residue 52 with aspartateThis study
pST428pRS416-HAA1-GFP, expressing HAA1-GFP from endogenous promoter, with GFP fused at C-terminal end of Haa1This study
pZL3210pRS416-HAA1-HA, expressing HAA1 from endogenous promoter with 3×HA epitope tag at C terminusThis study
pZL3338pRS415-HRR25-myc, expressing HRR25 from endogenous promoter with 3×myc epitope tag at C terminusThis study
pZL3361pRS415-HRR25(KD)-myc, expressing N-terminal kinase domain of Hrr25 (residues 1–302) from endogenous promoter with 3×myc epitope tag at C terminusThis study
pACTIIYeast 2-hybrid vector encoding GADClontech Laboratories, Inc.
pZL3539pRS415-TEF-GAD, with coding sequence of GAD from pACT-II cloned into XbaI site of pRS415-TEFThis study
pZL3554pRS415-TEF-GAD-HAA1, with HAA1 open reading frame cloned into BamHI and XhoI sites of pZL3539This study
pGBKT7Yeast 2-hybrid vector encoding GBDClontech Laboratories, Inc.
pZL3542pRS424-ADH1-GBD, with coding sequence of GBD from pGBKT7 cloned into XbaI site of pRS424-ADH1This study
pZL3557pRS424-ADH1-GBD-HRR25, with HRR25 open reading frame cloned into BamHI and SalI sites of pZL3542This study
pZL3736pRS424-ADH1-GBD-HRR25(KD), with DNA sequence encoding N-terminal kinase domain of Hrr25 (residues 1–302) cloned into BamHI and SalI sites of pZL3542
  • a GAD, Gal4 transcriptional activation domain; GBD, Gal4 DNA-binding domain.