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biofilm

  • YbeY Controls the Type III and Type VI Secretion Systems and Biofilm Formation through RetS in <span class="named-content genus-species" id="named-content-1">Pseudomonas aeruginosa</span>
    Genetics and Molecular Biology
    YbeY Controls the Type III and Type VI Secretion Systems and Biofilm Formation through RetS in Pseudomonas aeruginosa

    Pseudomonas aeruginosa causes a variety of acute and chronic infections in humans. The type III secretion system (T3SS) plays an important role in acute infection, and the type VI secretion system (T6SS) and biofilm formation are associated with chronic infections.

    Yushan Xia, Congjuan Xu, Dan Wang, Yuding Weng, Yongxin Jin, Fang Bai, Zhihui Cheng, Oscar P. Kuipers, Weihui Wu
  • A Viability Quantitative PCR Dilemma: Are Longer Amplicons Better?
    Genetics and Molecular Biology
    A Viability Quantitative PCR Dilemma: Are Longer Amplicons Better?

    Viability quantitative PCR (v-qPCR) has evolved into a valuable, mainstream technique for determining the number of viable microorganisms in samples by qPCR. Amplicon length is known to be positively correlated with the ability to distinguish between live and dead bacteria but is negatively correlated with qPCR efficiency.

    Wannes Van Holm, Justien Ghesquière, Nico Boon, Tim Verspecht, Kristel Bernaerts, Naiera Zayed, Ioanna Chatzigiannidou, Wim Teughels
  • <span class="named-content genus-species" id="named-content-1">Vibrio fischeri</span> Amidase Activity Is Required for Normal Cell Division, Motility, and Symbiotic Competence
    Microbial Ecology
    Vibrio fischeri Amidase Activity Is Required for Normal Cell Division, Motility, and Symbiotic Competence

    Peptidoglycan (PG) is a critical microbe-associated molecular pattern (MAMP) that is sloughed by cells of V. fischeri during symbiotic colonization of squid. Specifically, this process induces significant remodeling of a specialized symbiotic light organ within the squid mantle cavity.

    Pat M. Fidopiastis, Vanessa Mariscal, Jeanne-Marie McPherson, Sarah McAnulty, Anne Dunn, Eric V. Stabb, Karen L. Visick
  • <span class="named-content genus-species" id="named-content-1">Bacillus subtilis</span> Modulates Its Usage of Biofilm-Bound Iron in Response to Environmental Iron Availability
    Environmental Microbiology
    Bacillus subtilis Modulates Its Usage of Biofilm-Bound Iron in Response to Environmental Iron Availability

    Recent pieces of evidence suggest that Fe bound to the biofilm could assume at least two important functions, a local source of Fe for uptake and a support to extracellular metabolism, such as extracellular electron transfer. Our results show that B. subtilis can use biofilm-bound Fe for uptake only if it does not compromise Fe homeostasis of the biofilm, i.e.,...

    Adrien Rizzi, Julie Leroux, Vincent Charron-Lamoureux, Sébastien Roy, Pascale B. Beauregard, Jean-Philippe Bellenger
  • Thermoregulation of <em>Pseudomonas aeruginosa</em> Biofilm Formation
    Environmental Microbiology
    Thermoregulation of Pseudomonas aeruginosa Biofilm Formation

    Biofilm formation is an important protection mechanism used by most microorganisms and provides cells with many advantages, like high infectivity, antibiotic resistance, and strong survivability. Since most persistent bacterial infections are believed to be associated with biofilms, biofilm control is an important issue in medicine, environmental engineering, and industry. Biofilm formation is influenced by various environmental factors...

    Suran Kim, Xi-Hui Li, Hyeon-Ji Hwang, Joon-Hee Lee
  • Alterocin, an Antibiofilm Protein Secreted by <em>Pseudoalteromonas</em> sp. Strain 3J6
    Genetics and Molecular Biology | Spotlight
    Alterocin, an Antibiofilm Protein Secreted by Pseudoalteromonas sp. Strain 3J6

    Biofilms are microbial communities that develop on solid surfaces or interfaces and are detrimental in a number of fields, including for example food industry, aquaculture, and medicine. In the latter, antibiotics are insufficient to clear biofilm infections, leading to chronic infections such as in the case of infection by Pseudomonas aeruginosa of the lungs of...

    Albane Jouault, Angélique Gobet, Marjolaine Simon, Emilie Portier, Morgan Perennou, Erwan Corre, Fanny Gaillard, David Vallenet, Gurvan Michel, Yannick Fleury, Alexis Bazire, Alain Dufour
  • Genetic Determinants of <em>Salmonella</em> Resistance to the Biofilm-Inhibitory Effects of a Synthetic 4-Oxazolidinone Analog
    Genetics and Molecular Biology
    Genetic Determinants of Salmonella Resistance to the Biofilm-Inhibitory Effects of a Synthetic 4-Oxazolidinone Analog

    Biofilms are resistant to killing by disinfectants and antimicrobials. S. enterica biofilms facilitate long-term host colonization and persistence in food processing environments. Synthetic analogs of 4-oxazolidinone natural products show promise as antibiofilm agents. Here, we show that a synthetic 4-oxazolidinone analog inhibits Salmonella biofilm through...

    K. F. Griewisch, J. G. Pierce, J. R. Elfenbein
  • <span class="named-content genus-species" id="named-content-1">Staphylococcus aureus</span> Aggregates on Orthopedic Materials under Varying Levels of Shear Stress
    Environmental Microbiology | Spotlight
    Staphylococcus aureus Aggregates on Orthopedic Materials under Varying Levels of Shear Stress

    Periprosthetic joint infection occurring after artificial joint replacement is a major clinical issue that require repeated surgeries and antibiotic interventions. Unfortunately, 26% of patients die within 5 years of developing these infections. Staphylococcus aureus is the bacterium most commonly responsible for this problem and can form biofilms to provide...

    Tripti Thapa Gupta, Niraj K. Gupta, Matthew J. Pestrak, Devendra H. Dusane, Janette M. Harro, Alexander R. Horswill, Paul Stoodley
  • Antibiofilm Activity of <span class="named-content genus-species" id="named-content-1">Lactobacillus plantarum</span> 12 Exopolysaccharides against <span class="named-content genus-species" id="named-content-2">Shigella flexneri</span>
    Food Microbiology
    Antibiofilm Activity of Lactobacillus plantarum 12 Exopolysaccharides against Shigella flexneri

    S. flexneri is a widespread foodborne pathogen causing food contamination and responsible for food poisoning outbreaks related to various foods in developing countries. Not only has biofilm formation by S. flexneri been difficult to eliminate, but it has also increased the drug resistance of the...

    Yinglong Song, Mengying Sun, Lu Feng, Xue Liang, Xing Song, Guangqing Mu, Yanfeng Tuo, Shujuan Jiang, Fang Qian
  • Antibiofilm Activity of Small Molecules Produced by <span class="named-content genus-species" id="named-content-1">Staphylococcus epidermidis</span> against <span class="named-content genus-species" id="named-content-2">Staphylococcus aureus</span>
    Letter to the Editor
    Antibiofilm Activity of Small Molecules Produced by Staphylococcus epidermidis against Staphylococcus aureus
    Anjna Kumari, Rachna Singh

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