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Applied and Environmental Microbiology
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carbohydrate

  • Open Access
    <em>Trichoderma reesei</em> Dehydrogenase, a Pyrroloquinoline Quinone-Dependent Member of Auxiliary Activity Family 12 of the Carbohydrate-Active Enzymes Database: Functional and Structural Characterization
    Enzymology and Protein Engineering
    Trichoderma reesei Dehydrogenase, a Pyrroloquinoline Quinone-Dependent Member of Auxiliary Activity Family 12 of the Carbohydrate-Active Enzymes Database: Functional and Structural Characterization

    Pyrroloquinoline quinone (PQQ) is an important cofactor synthesized by prokaryotes and involved in enzymatic alcohol and sugar oxidation. In eukaryotes, the benefit of PQQ as a vitamin has been suggested but never proved. Recently, the first eukaryotic enzyme using PQQ was characterized in the basidiomycete Coprinopsis cinerea, demonstrating that fungi are able to use...

    Annick Turbe-Doan, Eric Record, Vincent Lombard, Rajender Kumar, Anthony Levasseur, Bernard Henrissat, Marie-Line Garron
  • Genes Involved in Galactooligosaccharide Metabolism in <span class="named-content genus-species" id="named-content-1">Lactobacillus reuteri</span> and Their Ecological Role in the Gastrointestinal Tract
    Physiology
    Genes Involved in Galactooligosaccharide Metabolism in Lactobacillus reuteri and Their Ecological Role in the Gastrointestinal Tract

    This study shows that lac genes in Lactobacillus reuteri encode hydrolases and transporters that are necessary for the metabolism of GOS, as well as α-galactoside substrates. Coculture experiments with the wild-type strain and a gos mutant clearly demonstrated that GOS utilization confers a growth advantage in medium containing GOS as the sole...

    Monchaya Rattanaprasert, Jan-Peter van Pijkeren, Amanda E. Ramer-Tait, Maria Quintero, Car Reen Kok, Jens Walter, Robert W. Hutkins
  • Enzymology and Protein Engineering
    A Novel Colletotrichum graminicola Raffinose Oxidase in the AA5 Family
    Martina Andberg, Filip Mollerup, Kirsti Parikka, Sanna Koutaniemi, Harry Boer, Minna Juvonen, Emma Master, Maija Tenkanen, Kristiina Kruus
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