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Applied and Environmental Microbiology
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Clostridium

  • Development of Strong Anaerobic Fluorescent Reporters for <span class="named-content genus-species" id="named-content-1">Clostridium acetobutylicum</span> and <span class="named-content genus-species" id="named-content-2">Clostridium ljungdahlii</span> Using HaloTag and SNAP-tag Proteins
    Methods
    Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins

    Up to this point, assays and methods involving fluorescent reporter proteins were unavailable or limited in Clostridium organisms and other strict anaerobes. Green fluorescent protein (GFP), mCherry, and flavin-binding proteins (and their derivatives) have been used only in a few clostridia with limited success and yielded low fluorescence compared to aerobic microbial systems. Recently, we reported a new strong fluorescent...

    Kamil Charubin, Hannah Streett, Eleftherios Terry Papoutsakis
  • Improved <em>n</em>-Butanol Production from <span class="named-content genus-species" id="named-content-1">Clostridium cellulovorans</span> by Integrated Metabolic and Evolutionary Engineering
    Biotechnology | Spotlight
    Improved n-Butanol Production from Clostridium cellulovorans by Integrated Metabolic and Evolutionary Engineering

    Due to a lack of genetic tools, Clostridium cellulovorans DSM 743B has not been comprehensively explored as a putative strain platform for n-butanol production by consolidated bioprocessing (CBP). Based on the previous study of genetic tools, strain engineering of C. cellulovorans for the...

    Zhiqiang Wen, Rodrigo Ledesma-Amaro, Jianping Lin, Yu Jiang, Sheng Yang
  • Physiology
    Identification of Bacterial Species That Can Utilize Fructose-Asparagine
    Anice Sabag-Daigle, Jikang Wu, Mikayla A. Borton, Anindita Sengupta, Venkat Gopalan, Kelly C. Wrighton, Vicki H. Wysocki, Brian M. M. Ahmer
  • Environmental Microbiology
    Evolution of a Biomass-Fermenting Bacterium To Resist Lignin Phenolics
    Tristan Cerisy, Tiffany Souterre, Ismael Torres-Romero, Magali Boutard, Ivan Dubois, Julien Patrouix, Karine Labadie, Wahiba Berrabah, Marcel Salanoubat, Volker Doring, Andrew C. Tolonen
  • Physiology
    Modulation of the Acetone/Butanol Ratio during Fermentation of Corn Stover-Derived Hydrolysate by Clostridium beijerinckii Strain NCIMB 8052
    Zi-Yong Liu, Xiu-Qing Yao, Quan Zhang, Zhen Liu, Ze-Jie Wang, Yong-Yu Zhang, Fu-Li Li
  • Biotechnology
    Adenine Addition Restores Cell Viability and Butanol Production in Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) Cultivated at 37°C
    Keiji Kiyoshi, Sohei Kawashima, Kosuke Nobuki, Toshimori Kadokura, Atsumi Nakazato, Ken-ichiro Suzuki, Shunichi Nakayama
  • Biotechnology
    Development of a High-Efficiency Transformation Method and Implementation of Rational Metabolic Engineering for the Industrial Butanol Hyperproducer Clostridium saccharoperbutylacetonicum Strain N1-4
    Nicolaus A. Herman, Jeffrey Li, Ripika Bedi, Barbara Turchi, Xiaoji Liu, Michael J. Miller, Wenjun Zhang
  • Microbial Ecology
    Microbial Community Development in a Dynamic Gut Model Is Reproducible, Colon Region Specific, and Selective for Bacteroidetes and Clostridium Cluster IX
    Pieter Van den Abbeele, Charlotte Grootaert, Massimo Marzorati, Sam Possemiers, Willy Verstraete, Philippe Gérard, Sylvie Rabot, Aurélia Bruneau, Sahar El Aidy, Muriel Derrien, Erwin Zoetendal, Michiel Kleerebezem, Hauke Smidt, Tom Van de Wiele
  • Enzymology and Protein Engineering
    Engineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability
    Wenjin Liu, Xiao-Zhou Zhang, Zuoming Zhang, Y.-H. Percival Zhang
  • Public Health Microbiology
    Sequence Diversity of Genes Encoding Botulinum Neurotoxin Type F
    Brian H. Raphael, Mallory J. Choudoir, Carolina Lúquez, Rafael Fernández, Susan E. Maslanka

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