Clostridium
- MethodsDevelopment of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins
Up to this point, assays and methods involving fluorescent reporter proteins were unavailable or limited in Clostridium organisms and other strict anaerobes. Green fluorescent protein (GFP), mCherry, and flavin-binding proteins (and their derivatives) have been used only in a few clostridia with limited success and yielded low fluorescence compared to aerobic microbial systems. Recently, we reported a new strong fluorescent...
- Biotechnology | SpotlightImproved n-Butanol Production from Clostridium cellulovorans by Integrated Metabolic and Evolutionary Engineering
Due to a lack of genetic tools, Clostridium cellulovorans DSM 743B has not been comprehensively explored as a putative strain platform for n-butanol production by consolidated bioprocessing (CBP). Based on the previous study of genetic tools, strain engineering of C. cellulovorans for the...
- Environmental MicrobiologyEvolution of a Biomass-Fermenting Bacterium To Resist Lignin Phenolics
- Enzymology and Protein EngineeringEngineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability
- Public Health MicrobiologySequence Diversity of Genes Encoding Botulinum Neurotoxin Type F