Clostridium acetobutylicum

Biotechnology
A Strongly Fluorescing Anaerobic Reporter and Protein-Tagging System for Clostridium Organisms Based on the Fluorescence-Activating and Absorption-Shifting Tag Protein (FAST)
FAST in association with the fluorogenic ligand HMBR is characterized as a successful, highly fluorescent reporter system in C. acetobutylicum. FAST can be used to distinguish between promoters in live cells using flow cytometry or a fluorescence microplate reader and can be used to tag and examine protein localization in live, anaerobically grown cells. Given that...

Hannah E. Streett, Katie M. Kalis, Eleftherios T. Papoutsakis
Biotechnology
Small and Low but Potent: the Complex Regulatory Role of the Small RNA SolB in Solventogenesis in Clostridium acetobutylicum
Alexander J. Jones, Alan G. Fast, Michael Clupper, Eleftherios T. Papoutsakis
Genetics and Molecular Biology
A Novel Dual-cre Motif Enables Two-Way Autoregulation of CcpA in Clostridium acetobutylicum
Lu Zhang, Yanqiang Liu, Yunpeng Yang, Weihong Jiang, Yang Gu
Biotechnology | Spotlight
Functional Expression of the Clostridium ljungdahlii Acetyl-Coenzyme A Synthase in Clostridium acetobutylicum as Demonstrated by a Novel In Vivo CO Exchange Activity En Route to Heterologous Installation of a Functional Wood-Ljungdahl Pathway
Alan G. Fast, Eleftherios T. Papoutsakis
Biotechnology
Heterologous Expression of the Clostridium carboxidivorans CO Dehydrogenase Alone or Together with the Acetyl Coenzyme A Synthase Enables both Reduction of CO₂ and Oxidation of CO by Clostridium acetobutylicum
Ellinor D. Carlson, Eleftherios T. Papoutsakis
Enzymology and Protein Engineering
Tertiary Structure and Characterization of a Glycoside Hydrolase Family 44 Endoglucanase from Clostridium acetobutylicum
Christopher D. Warner, Julie A. Hoy, Taran C. Shilling, Michael J. Linnen, Nathaniel D. Ginder, Clark F. Ford, Richard B. Honzatko, Peter J. Reilly
PHYSIOLOGY AND BIOTECHNOLOGY
O₂ and Reactive Oxygen Species Detoxification Complex, Composed of O₂-Responsive NADH:Rubredoxin Oxidoreductase-Flavoprotein A2-Desulfoferrodoxin Operon Enzymes, Rubperoxin, and Rubredoxin, in Clostridium acetobutylicum
Shinji Kawasaki, Yu Sakai, Tohru Takahashi, Ippei Suzuki, Youichi Niimura
Methods
Development and Application of Flow-Cytometric Techniques for Analyzing and Sorting Endospore-Forming Clostridia
Bryan P. Tracy, Stefan M. Gaida, Eleftherios T. Papoutsakis
Methods
A General Framework for Designing and Validating Oligomer-Based DNA Microarrays and Its Application to Clostridium acetobutylicum
Carlos J. Paredes, Ryan S. Senger, Iwona S. Spath, Jacob R. Borden, Ryan Sillers, Eleftherios T. Papoutsakis
Evolutionary and Genomic Microbiology
Dynamics of Genomic-Library Enrichment and Identification of Solvent Tolerance Genes for Clostridium acetobutylicum
Jacob R. Borden, Eleftherios Terry Papoutsakis

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