Clostridium difficile
Public and Environmental Health MicrobiologyHigh Prevalence of Clostridium difficile in Home Gardens in Western AustraliaRecently, community-associated Clostridium difficile infection (CA-CDI) has emerged as a significant problem, accounting for ∼50% of all CDI cases and reported to affect a younger population without traditional risk factors. Possible sources of CA-CDI are soil, food, and water contaminated by animal feces, and recent reports show overlapping ribotypes of...
Genetics and Molecular Biology | SpotlightUsing an Endogenous CRISPR-Cas System for Genome Editing in the Human Pathogen Clostridium difficileClostridium difficile represents today a real danger for human and animal health. It is the leading cause of diarrhea associated with health care in adults in industrialized countries. The incidence of these infections continues to increase, and this trend is accentuated by the general aging of the population. Many questions about the mechanisms contributing to...
Public and Environmental Health MicrobiologyBiocide Resistance and Transmission of Clostridium difficile Spores Spiked onto Clinical Surfaces from an American Health Care FacilityClostridium difficile is a health care-acquired organism and the causative agent of antibiotic-associated diarrhea. Its spores are implicated in fecal to oral transmission from contaminated surfaces in the health care environment due to their adherent nature. Contaminated surfaces are cleaned using high-strength chemicals to remove and kill the spores; however,...
MethodsGeneration of Markerless Deletions in the Nosocomial Pathogen Clostridium difficile by Induction of DNA Double-Strand BreaksMost sequenced bacterial genomes contain genes encoding proteins of unknown or hypothetical function. To identify a phenotype for mutations in such genes, deletion is the preferred method for mutagenesis because it reduces the likelihood of polar effects, although it does not eliminate the possibility. Allelic exchange to produce deletions is dependent on the length of homologous regions used to generate merodiploids. Shorter regions of...
- Public Health MicrobiologyUse of Purified Clostridium difficile Spores To Facilitate Evaluation of Health Care Disinfection Regimens
- Genetics and Molecular BiologyA mariner-Based Transposon System for In Vivo Random Mutagenesis of Clostridium difficile
- Genetics and Molecular BiologyMorphological and Genetic Diversity of Temperate Phages in Clostridium difficile