coupled-enzyme system
BiotechnologySimultaneous Directed Evolution of Coupled Enzymes for Efficient Asymmetric Synthesis of l-PhosphinothricinThe traditional strategy to improve multienzyme-catalyzed reaction efficiencies may lead to enhanced single-enzyme catalytic efficiencies but may also result in the loss of coordination among enzymes. We describe a directed evolution strategy for an entire coupled-enzyme system to simultaneously enhance enzyme coordination and catalytic efficiency.