CRISPR
Genetics and Molecular Biology | SpotlightUsing an Endogenous CRISPR-Cas System for Genome Editing in the Human Pathogen Clostridium difficileClostridium difficile represents today a real danger for human and animal health. It is the leading cause of diarrhea associated with health care in adults in industrialized countries. The incidence of these infections continues to increase, and this trend is accentuated by the general aging of the population. Many questions about the mechanisms contributing to...
Minireview | SpotlightDeterminants of Phage Host Range in Staphylococcus SpeciesBacteria in the genus Staphylococcus are important targets for phage therapy due to their prevalence as pathogens and increasing antibiotic resistance. Here we review Staphylococcus outer surface features and specific phage resistance mechanisms that define the host range, the set of strains that an individual phage can potentially infect.
MethodsCRISPR-Cas9 and CRISPR-Assisted Cytidine Deaminase Enable Precise and Efficient Genome Editing in Klebsiella pneumoniaeGenetics is a key means to study bacterial physiology. However, the highly desirable scarless genetic manipulation is often time-consuming and laborious for the major human pathogen K. pneumoniae. We developed a CRISPR-Cas9-mediated genome-editing method and a cytidine base-editing system, enabling rapid, highly efficient, and iterative genome editing in both...
Food Microbiology | SpotlightHigh-Resolution Identification of Multiple Salmonella Serovars in a Single Sample by Using CRISPR-SeroSeqSalmonella enterica is the leading bacterial cause of foodborne illness in the United States and is represented by over 2,600 distinct serovars. Some of these serovars are pathogenic in humans, while others are not. Current surveillance for this pathogen is limited by the detection of only the most abundant serovars, due to the culture-based approaches that are used....
- MethodsCRISPR-Cpf1-Assisted Multiplex Genome Editing and Transcriptional Repression in Streptomyces
Rapid, efficient genetic engineering of Streptomyces strains is critical for genome mining of novel natural products (NPs) as well as strain improvement. Here, a novel and high-efficiency Streptomyces genome editing tool is established based on the FnCRISPR-Cpf1 system, which is an...
- Environmental MicrobiologyThe Enigmatic Genome of an Obligate Ancient Spiroplasma Symbiont in a Hadal Holothurian
- Genetics and Molecular BiologyCRISPR-Cas9D10A Nickase-Assisted Genome Editing in Lactobacillus casei