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Applied and Environmental Microbiology
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DNA sequencing

  • Methicillin-Resistant <span class="named-content genus-species" id="named-content-1">Staphylococcus aureus</span> Strains in Swiss Pigs and Their Relation to Isolates from Farmers and Veterinarians
    Public and Environmental Health Microbiology
    Methicillin-Resistant Staphylococcus aureus Strains in Swiss Pigs and Their Relation to Isolates from Farmers and Veterinarians

    MRSA is an important human pathogen; thus, its increasing prevalence in livestock over the last decade has a potentially large impact on public health. Farmers and veterinarians are especially at risk due to their close contact with animals. Our work demonstrates a dramatic increase in MRSA prevalence in Swiss pigs, from 2% in 2009 to 44% in 2017. Whole-genome sequencing allowed us to show a close association between farmer and pig...

    Sonja Kittl, Isabelle Brodard, Dagmar Heim, Patrizia Andina-Pfister, Gudrun Overesch
  • The Future of Environmental DNA in Forensic Science
    Minireview | Spotlight
    The Future of Environmental DNA in Forensic Science

    DNA sequencing technologies continue to improve, and there has been a corresponding expansion of DNA-based applications in the forensic sciences. DNA recovered from dust and environmental debris can be used to identify the organisms associated with these sample types, including bacteria, plants, fungi, and insects. Such results can then be leveraged to discern sample origin or geolocation and investigate individual identification.

    ...
    Julia S. Allwood, Noah Fierer, Robert R. Dunn
  • Use of a Fluorescent Analog of Glucose (2-NBDG) To Identify Uncultured Rumen Bacteria That Take Up Glucose
    Environmental Microbiology
    Use of a Fluorescent Analog of Glucose (2-NBDG) To Identify Uncultured Rumen Bacteria That Take Up Glucose

    We introduce a method for identifying what substrates are consumed by bacteria in natural communities. Our method offers significant improvement over existing methods for studying this characteristic. Our method uses a fluorescently labeled substrate which clearly labels target bacteria (glucose consumers in our case). Previous methods use isotope-labeled substrates, which are notorious for off-target labeling (due to cross-feeding of...

    Junyi Tao, Courtney McCourt, Halima Sultana, Corwin Nelson, John Driver, Timothy J. Hackmann
  • Mesophilic Sporeformers Identified in Whey Powder by Using Shotgun Metagenomic Sequencing
    Methods
    Mesophilic Sporeformers Identified in Whey Powder by Using Shotgun Metagenomic Sequencing

    The ability of sporeformers to remain dormant in a desiccated state is of concern from a safety and spoilage perspective in dairy powder. Traditional culturing techniques are slow and provide little information without further investigation. We describe the identification of mesophilic sporeformers present in powders produced over 1 year, using novel shotgun metagenomic sequencing. This method allows detection and identification of...

    Aoife J. McHugh, Conor Feehily, John T. Tobin, Mark A. Fenelon, Colin Hill, Paul D. Cotter
  • Open Access
    Minireview
    The Madness of Microbiome: Attempting To Find Consensus “Best Practice” for 16S Microbiome Studies
    Jolinda Pollock, Laura Glendinning, Trong Wisedchanwet, Mick Watson
  • Minireview | Spotlight
    The Emergency Medical Service Microbiome
    Andrew J. Hudson, Graeme D. Glaister, Hans-Joachim Wieden
  • Evolutionary and Genomic Microbiology
    Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains
    Yong-Biao Huo, Yuki Chan, Donnabella C. Lacap-Bugler, Sisu Mo, Patrick C. Y. Woo, W. Keung Leung, Rory M. Watt
  • Environmental Microbiology
    Allelochemicals Produced by Brown Macroalgae of the Lobophora Genus Are Active against Coral Larvae and Associated Bacteria, Supporting Pathogenic Shifts to Vibrio Dominance
    Kathleen M. Morrow, Katrina Bromhall, Cherie A. Motti, Colin B. Munn, David G. Bourne
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