DNA sequencing
Environmental MicrobiologyUse of a Fluorescent Analog of Glucose (2-NBDG) To Identify Uncultured Rumen Bacteria That Take Up GlucoseWe introduce a method for identifying what substrates are consumed by bacteria in natural communities. Our method offers significant improvement over existing methods for studying this characteristic. Our method uses a fluorescently labeled substrate which clearly labels target bacteria (glucose consumers in our case). Previous methods use isotope-labeled substrates, which are notorious for off-target labeling (due to cross-feeding of...
MethodsMesophilic Sporeformers Identified in Whey Powder by Using Shotgun Metagenomic SequencingThe ability of sporeformers to remain dormant in a desiccated state is of concern from a safety and spoilage perspective in dairy powder. Traditional culturing techniques are slow and provide little information without further investigation. We describe the identification of mesophilic sporeformers present in powders produced over 1 year, using novel shotgun metagenomic sequencing. This method allows detection and identification of...
- Minireview | SpotlightThe Emergency Medical Service Microbiome
- Evolutionary and Genomic MicrobiologyMultilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains