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Applied and Environmental Microbiology
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gene deletion

  • Using the Endogenous CRISPR-Cas System of <em>Heliobacterium modesticaldum</em> To Delete the Photochemical Reaction Center Core Subunit Gene
    Methods | Spotlight
    Using the Endogenous CRISPR-Cas System of Heliobacterium modesticaldum To Delete the Photochemical Reaction Center Core Subunit Gene

    The heliobacteria are the only phototrophic members of the largely Gram-positive phylum Firmicutes, which contains medically and industrially important members, such as Clostridium difficile and Clostridium acetobutylicum. Heliobacteria are of interest in the study of photosynthesis because...

    Patricia L. Baker, Gregory S. Orf, Kimberly Kevershan, Michael E. Pyne, Taner Bicer, Kevin E. Redding
  • Boosting Secretion of Extracellular Protein by <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> via Cell Wall Perturbation
    Enzymology and Protein Engineering
    Boosting Secretion of Extracellular Protein by Escherichia coli via Cell Wall Perturbation

    Cell surface structure stabilization is important for extracellular secretion of proteins in Escherichia coli. As the main constituent of the cell wall, peptidoglycan contributes to cell structure robustness and stability. Here, we perturbed the peptidoglycan network by deleting dacA and dacB genes encoding d,...

    Haiquan Yang, Xiao Lu, Jinyuan Hu, Yuan Chen, Wei Shen, Long Liu
  • Environmental Microbiology
    Diazotrophic Growth Allows Azotobacter vinelandii To Overcome the Deleterious Effects of a glnE Deletion
    Florence Mus, Alex Tseng, Ray Dixon, John W. Peters
  • Evolutionary and Genomic Microbiology
    Adaptive Evolution of Extreme Acidophile Sulfobacillus thermosulfidooxidans Potentially Driven by Horizontal Gene Transfer and Gene Loss
    Xian Zhang, Xueduan Liu, Yili Liang, Xue Guo, Yunhua Xiao, Liyuan Ma, Bo Miao, Hongwei Liu, Deliang Peng, Wenkun Huang, Yuguang Zhang, Huaqun Yin
  • Genetics and Molecular Biology
    Development of an Improved System for the Generation of Knockout Mutants of Amycolatopsis sp. Strain ATCC 39116
    Florian Meyer, Hilke Pupkes, Alexander Steinbüchel
  • Biotechnology
    Deletion of Genes Encoding Cytochrome Oxidases and Quinol Monooxygenase Blocks the Aerobic-Anaerobic Shift in Escherichia coli K-12 MG1655
    Vasiliy A. Portnoy, David A. Scott, Nathan E. Lewis, Yekaterina Tarasova, Andrei L. Osterman, Bernhard Ø. Palsson
  • Methods
    Development of pyrF-Based Genetic System for Targeted Gene Deletion in Clostridium thermocellum and Creation of a pta Mutant
    Shital A. Tripathi, Daniel G. Olson, D. Aaron Argyros, Bethany B. Miller, Trisha F. Barrett, Daniel M. Murphy, Jesse D. McCool, Anne K. Warner, Vineet B. Rajgarhia, Lee R. Lynd, David A. Hogsett, Nicky C. Caiazza
  • Biotechnology
    Identification and Elimination of the Competing N-Acetyldiaminopentane Pathway for Improved Production of Diaminopentane by Corynebacterium glutamicum
    Stefanie Kind, Weol Kyu Jeong, Hartwig Schröder, Oskar Zelder, Christoph Wittmann
  • Biotechnology
    Metabolic Engineering of Gluconobacter oxydans for Improved Growth Rate and Growth Yield on Glucose by Elimination of Gluconate Formation
    Vera Krajewski, Petra Simić, Nigel J. Mouncey, Stephanie Bringer, Hermann Sahm, Michael Bott
  • Biotechnology
    Lactococcus lactis fabH, Encoding β-Ketoacyl-Acyl Carrier Protein Synthase, Can Be Functionally Replaced by the Plasmodium falciparum Congener
    Yu Du, Jolyn E. Gisselberg, Jacob D. Johnson, Patricia J. Lee, Sean T. Prigge, Brian O. Bachmann

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