gene deletion
Methods | SpotlightUsing the Endogenous CRISPR-Cas System of Heliobacterium modesticaldum To Delete the Photochemical Reaction Center Core Subunit GeneThe heliobacteria are the only phototrophic members of the largely Gram-positive phylum Firmicutes, which contains medically and industrially important members, such as Clostridium difficile and Clostridium acetobutylicum. Heliobacteria are of interest in the study of photosynthesis because...
Enzymology and Protein EngineeringBoosting Secretion of Extracellular Protein by Escherichia coli via Cell Wall PerturbationCell surface structure stabilization is important for extracellular secretion of proteins in Escherichia coli. As the main constituent of the cell wall, peptidoglycan contributes to cell structure robustness and stability. Here, we perturbed the peptidoglycan network by deleting dacA and dacB genes encoding d,...
- Environmental MicrobiologyDiazotrophic Growth Allows Azotobacter vinelandii To Overcome the Deleterious Effects of a glnE Deletion
- Evolutionary and Genomic MicrobiologyAdaptive Evolution of Extreme Acidophile Sulfobacillus thermosulfidooxidans Potentially Driven by Horizontal Gene Transfer and Gene Loss
- Genetics and Molecular BiologyDevelopment of an Improved System for the Generation of Knockout Mutants of Amycolatopsis sp. Strain ATCC 39116