Gene Knockout Techniques

Genetics and Molecular Biology
Use of Natural Transformation To Establish an Easy Knockout Method in Riemerella anatipestifer
MaFeng Liu, Li Zhang, Li Huang, Francis Biville, DeKang Zhu, MingShu Wang, RenYong Jia, Shun Chen, KunFeng Sun, Qiao Yang, Ying Wu, XiaoYue Chen, AnChun Cheng
Genetics and Molecular Biology
Development of an Improved System for the Generation of Knockout Mutants of Amycolatopsis sp. Strain ATCC 39116
Florian Meyer, Hilke Pupkes, Alexander Steinbüchel
Genetics and Molecular Biology
Genetic Transformation and Mutagenesis via Single-Stranded DNA in the Unicellular, Diazotrophic Cyanobacteria of the Genus Cyanothece
Hongtao Min, Louis A. Sherman
Methods
Development of pyrF-Based Genetic System for Targeted Gene Deletion in Clostridium thermocellum and Creation of a pta Mutant
Shital A. Tripathi, Daniel G. Olson, D. Aaron Argyros, Bethany B. Miller, Trisha F. Barrett, Daniel M. Murphy, Jesse D. McCool, Anne K. Warner, Vineet B. Rajgarhia, Lee R. Lynd, David A. Hogsett, Nicky C. Caiazza
Methods
Targeted Chromosomal Knockouts in Mycoplasma pneumoniae
Radha Krishnakumar, Nacyra Assad-Garcia, Gwynedd A. Benders, Quang Phan, Michael G. Montague, John I. Glass
Genetics and Molecular Biology
Facilitation of Direct Conditional Knockout of Essential Genes in Bacillus licheniformis DSM13 by Comparative Genetic Analysis and Manipulation of Genetic Competence
Kerstin Hoffmann, Antje Wollherr, Michael Larsen, Michael Rachinger, Heiko Liesegang, Armin Ehrenreich, Friedhelm Meinhardt
Genetics and Molecular Biology
Enhanced Production and Secretion of Heterologous Proteins by the Filamentous Fungus Aspergillus oryzae via Disruption of Vacuolar Protein Sorting Receptor Gene Aovps10
Jaewoo Yoon, Tuerxun Aishan, Jun-ichi Maruyama, Katsuhiko Kitamoto
Physiology
Construction and Characterization of Three Lactate Dehydrogenase-Negative Enterococcus faecalis V583 Mutants
Maria Jönsson, Zhian Saleihan, Ingolf F. Nes, Helge Holo
Methods
Construction of a Gene Knockout System for Application in Paenibacillus alvei CCM 2051^T, Exemplified by the S-Layer Glycan Biosynthesis Initiation Enzyme WsfP
Kristof Zarschler, Bettina Janesch, Sonja Zayni, Christina Schäffer, Paul Messner